Microbial metabolism and enzyme function

Diverse and novel lanthanide-binding PQQ-dependent enzymes

The paper identifies Ln-dependent PQQ-dependent enzymes in bacteria, showing they are widespread and involved in carbon metabolism.

Adaptation of Methylobacterium extorquens to alternating carbon sources identifies the regulator CstR as an intersectional hub of cellular carbon metabolic dynamics and stress response

This study identifies CstR as a key regulator in Methylobacterium extorquens that coordinates carbon source transitions and stress responses, with loss-of-function mutations improving metabolic flexibility but reducing stress resilience.

Engineering Methyl-Coenzyme M Reductase for Enhanced Methane and Carbon Dioxide Capture through Biofilm Growth

This paper engineers methyl-coenzyme M reductase (Mcr) to enhance methane and carbon dioxide capture by identifying beneficial substitutions that improve biofilm growth and methane capture.

PqqU (PA2289) is responsible for Pyrroloquinoline Quinone Uptake in Pseudomonas aeruginosa

This study identifies PqqU as the primary PQQ importer in P. aeruginosa, showing its essential role in PQQ uptake and metabolic adaptability.

Decreasing peptide deformylase activity is a beneficial strategy for increasing formaldehyde resistance in Methylobacterium extorquens

This study shows that reducing peptide deformylase activity in Methylobacterium extorquens increases formaldehyde resistance, with pleiotropic effects and tradeoffs in heat shock resistance.

Adaptation of the freshwater anaerobic methanotroph 'Ca. Methanoperedens vercellensis' to low pH levels reveals membrane lipid remodelling

This study shows that Ca. Methanoperedens vercellensis adapts to low pH by remodelling membrane lipids, maintaining methane oxidation activity despite increased energy demands.

Anaerobic isoprene reduction by Pelotomaculum sp. from Eucalyptus-leaf sediments and its impacts on methanogenesis

This study identifies anaerobic isoprene reduction by Pelotomaculum sp. in Eucalyptus-leaf sediments and shows it inhibits methanogenesis by competing for hydrogen.

Non-enzymatic assimilation of organosulfur compounds at the interface of geochemistry and biochemistry

This study shows that microbial assimilation of DMS and DMSO can occur through non-enzymatic oxidative mechanisms driven by light and heat, producing methanesulfonic acid and sulfite.

A national baseline for methane sink habitats and methanotroph diversity

The paper presents a national study of methanotroph diversity and distribution in Denmark, revealing uncharacterised species as dominant in natural ecosystems and highlighting potential targets for methane mitigation.

Isolation of Crenothrix bacteria reveals the distinct ecophysiologies of filamentous methanotrophs and adaptations to redox stress

This study isolates two Crenothrix methanotroph strains, revealing their microaerobic metabolism, filamentous structure, and adaptations to redox stress, with implications for global methane cycling.

Direct carbon monoxide fixation via the bacterial and archaeal Wood-Ljungdahl pathways

The paper shows direct CO fixation to acetyl-CoA in archaeal and bacterial Wood-Ljungdahl pathways under hydrogenogenic growth using 13C tracer-based metabolomics.

Ectoine production by newly isolated halophilic methanotroph Methylotuvimicrobium percolatum using surplus methane from anaerobic digestion

This study isolates a halophilic methanotroph capable of converting surplus methane into ectoine, a high-value compound, under optimized conditions.

Genomic, physiological, and transcriptomic mechanisms of pH stress adaptation and response in Methylocystis sumavensis, a novel peatland methanotroph

This study identifies genomic, physiological, and transcriptomic mechanisms enabling Methylocystis sumavensis to adapt to pH stress in peatlands.

Evolutionary genomics reveals plant-associated origins of acetic acid bacteria in fermented food

This study traces the evolutionary origins of acetic acid bacteria, showing they transitioned from plant-associated lifestyles to fermented foods, not from social insect hosts.

Direct HPLC Method for Reduced Pyrroloquinoline Quinone in Functional and Biological Matrices

This paper develops an HPLC method to accurately quantify reduced PQQ in complex matrices and biological samples.

Unraveling and overcoming the ammonia toxicity in methanotrophs for sustainable biomanufacturing and methane removal

This study identifies a porin gene that facilitates ammonium uptake in methanotrophs, reduces ammonia toxicity and N2O production, and improves the sustainability of using ammonium as a nitrogen source in biomanufacturing.

Differences in Methanotrophic Community Structure in Two Methane-Rich Habitats: Oil Natural Gas Field & Paddy Field

The paper identifies and characterizes two methanotrophic bacterial isolates from different methane-rich habitats, showing sequence homology and morphological differences.