Discover, rate, and discuss biology preprints.

This paper develops a high-throughput method to screen cryoprotective agents for membrane permeability and toxicity, identifying 23 compounds with favorable properties.

This study develops antimicrobial scaffolds to protect BM-MSC-based implants from bacterial infections, showing effective inhibition of bacterial growth and enhanced bone regeneration in vivo.

The paper describes a low-cost Arduino-based organ perfusion system for rat liver studies, capable of simulating portal flow and controlling temperature and flow rates for ex-vivo experiments.

This study develops an in vitro lymph node microphysiological system to model early T cell-B cell interactions and antibody responses using primary human lymphocytes.

The paper screens 2,418 kinase inhibitors across five human cell types to identify cell-type specific contractility responses and challenges assumptions about pathway conservation.

This paper presents a de novo design strategy to convert the dimeric enzyme FAcD into an active monomer, achieving high catalytic activity with a mutant showing improved thermal stability.

This study optimizes heterologous expression and fermentation conditions to produce ikarugamycin at high yields, making it more affordable for biomedical research.

This paper develops a genetic switch for Lactiplantibacillus plantarum using a bacteriophage promoter and cumate inducer, and shows that encapsulation in alginate reduces leaky expression and improves switch performance.

This study uses antibody repertoire data and machine learning to predict and engineer antibody affinities, achieving accurate predictions and successful in silico design of seven out of eight synthetic variants.

This paper introduces a drop millifluidic system to screen and sort multicellular aggregates by pheno-morphological traits, enabling efficient analysis for drug testing.

EV-CLIP detects tumor-derived extracellular vesicle RNAs directly from blood plasma with high sensitivity, enabling precise monitoring of cancer mutations and EV subpopulations.

This study engineers and decellularizes human cartilage grafts, demonstrating their immuno-evasive properties and ability to repair skeletal defects in rats.

This paper develops a prodrug system for targeted colonic delivery of budesonide using gut commensals, showing improved drug localization and reduced systemic side effects.

This study shows perivascular cells sense mechanical changes in collagen matrix, affecting vascular function and contributing to fibrotic conditions.

This study investigates how GBM influences microglia invasion through contractility, independent of MMP activity and glycolysis, using microfluidic systems and 3D collagen hydrogels.

This paper develops a carboxylated graphene-based biosensor for detecting IgA-SARS-CoV-2 antibodies with a limit of detection of 1:1601 v/v.

This study uses CRISPR base editing to introduce revertant mutations in F508del-CFTR, rescuing protein folding and function in CF patient-derived cells.

This paper investigates how the size of gold nanoparticle contrast agents affects CT imaging of the gastrointestinal tract in ulcerative colitis.

The paper investigates the pathological mechanisms of renal biomineralization using patient samples and organ-chip devices, identifying key pathways and cellular changes associated with stone formation.

This study visualizes lipid nanoparticle trafficking in non-human primates using PET imaging, showing distribution to muscle and draining lymph nodes with uptake by antigen-presenting cells.