Discover, rate, and discuss biology preprints.

Pan-human Azimuth maps diverse human cells into a unified hierarchical typology, enabling cross-tissue comparisons and spatial transcriptomic annotation.

This study examines sex chromosome evolution in beetles using genome assemblies, finding conserved X regions, variable Y chromosomes, and independent neo-X and neo-Y formation.

This paper reports the annotation of three tiger beetle genomes, providing draft reference genomes for evolutionary and conservation studies.

This paper develops a DNA extraction protocol for museum-grade insects, enabling long-read sequencing, phylogenetics, and methylation profiling from degraded samples.

The paper evaluates EV-associated nucleic acids and proteins as a complementary method to eDNA/eRNA for biodiversity monitoring and physiological signal recovery in aquatic environments.

This paper introduces spRandom-seq, a spatial RNA sequencing method for FFPE tissues, which captures both host and microbial RNAs with higher efficiency than oligo(dT)-based methods.

The paper assesses genetic diversity and relatedness in sexually produced coral offspring to inform restoration strategies.

The paper develops an integrative framework to classify and interpret promoter-centric chromatin interactions by combining Hi-C, ChIA-PET, and functional genomic data, identifying a distinct class of architectural promoter-enhancer interactions with context-dependent regulatory roles.

PanvaR integrates GWAS and fine-mapping in one tool, providing LD analysis, SNP effect predictions, and candidate gene lists with visualizations.

This study identifies ferroptosis-related biomarkers in early-onset Alzheimer's disease using machine learning and develops a diagnostic model with high AUC-ROC values.

The paper develops a method to create high-resolution single-molecule replication maps of the human genome using multipulse BrdU labelling and ForkML.

This paper introduces UURNA-seq, a high-throughput single-nucleus RNA sequencing method, and constructs a comprehensive obesity atlas revealing transcriptional changes and metabolic pathway dysregulation.

This study shows that Uhrf1 loss causes DNA methylation loss and chromatin disruption in mouse embryos, leading to developmental failure and altered lineage specification.

The paper identifies epigenetic differences between HPV-human hybrid and human-only ecDNAs in cervical cancer, showing allele-specific regulation and enhancer activity in hybrid ecDNAs.

The paper describes the gene model for the ortholog of tgo in Drosophila persimilis using a specific gene annotation protocol.

The paper introduces easySHARE-seq, a method for simultaneous single-cell ATAC-seq and RNA-seq, showing improved transcript recovery and scalability in murine liver nuclei.

This study shows histone hyperacetylation disrupts TAD intra-interactions but preserves CTCF-CTCF loops, revealing distinct cohesin populations with different sensitivity to acetylation.

The paper maps concealed mitochondrial DNA mutations across 2.7 million cells from 311 individuals, finding that low-prevalence mutations accumulate with age and predict human age.

The paper identifies non-B DNA structures as the cause of sequencing bias against simple satellite DNA in long-read sequencing and suggests solutions to mitigate this bias.

The paper evaluates demographic representation in single-cell omic atlases and finds significant over-representation of European populations and under-representation of Asian and Latino individuals.