Author

A. Akiva

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 2 Bioengineering· 2 Biophysics· 2 Cell Biology· 2 Microbiology· 2

Signature Assays, Organisms & Methods

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Approach:experimental assay· 10 Research Focus:cell type annotation· 5 Assay:electronic health records· 3 Assay:imaging· 3 Research Focus:development & morphogenesis· 2 Organism:human· 2 Research Focus:cell signaling & trafficking· 1 Research Focus:cell-cell communication· 1

Frequent Co-Authors

N. Sommerdijk· 7 shared R. Roverts· 5 shared L. Rutten· 4 shared D. Daviran· 3 shared M. de Beer· 3 shared M. Kea-te Lindert· 3 shared E. Macias-Sanchez· 2 shared J. M. Schaart· 2 shared

Activity by Year

2020-2023 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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7 shared papersCell Biology

N. Sommerdijk

Mutual co-authors in cluster

Author profile →

10 papers

Recent·Most cited

Osteoblast-Induced Collagen Alignment in a 3D in vitro Bone Model

J. M. Schaart, M. Kea-te Lindert, R. Roverts, W. H. Nijhuis, N. Sommerdijk, A. Akiva

This study develops a 3D in vitro bone model to show osteoblasts organize collagen matrix alignment during early osteogenesis without osteoclast activity.

bioRxiv · Nov 17, 2023Cell Biology

Unravelling the relationship between isolated bone matrix vesicles and forming mineral at the nanometer scale

M. A. Eufrasio Cruz, L. Rutten, M. Martens, O. Arntz, F. van de Loo, E. Macias Sanchez, A. Akiva, P. Ciancaglini, A. P. Ramos, N. Sommerdijk

This study investigates the role of matrix vesicles in bone mineralization using cryoTEM and bulk analysis, revealing that mineralization is primarily driven by phosphatase activity with non-vesicular components playing a secondary role.

bioRxiv · May 9, 2023 · PreprintBiophysics

A Cryo-to-Liquid Phase Correlative Light Electron Microscopy Workflow for the Visualization of Biological Processes in Graphene Liquid Cells

L. Rutten, B. Joosten, J. M. Schaart, M. d. Beer, R. Roverts, S. Graber, W. Jahnen-Dechent, A. Akiva, E. Macias-Sanchez, N. Sommerdijk

This paper introduces a cryo-to-liquid phase correlative light/electron microscopy workflow to overcome limitations of graphene liquid cells and enables imaging of biological processes.

bioRxiv · May 8, 2023Biochemistry

Comparative 3D ultrastructure of Plasmodium falciparum gametocytes

F. Evers, R. Roverts, C. Boshoven, M. Kea-te Lindert, J. M. Verhoef, R. E. Sinden, A. Akiva, T. W. A. Kooij

This study uses high-resolution electron microscopy to reveal detailed 3D ultrastructure of Plasmodium falciparum gametocytes, challenging previous assumptions about mitochondria and organelle interactions.

bioRxiv · Mar 10, 2023 · PreprintMicrobiology

A 3D cell-free bone model shows collagen mineralization is driven and controlled by the matrix

R. H. M. Van der Meijden, D. Daviran, L. Rutten, F. Walboomers, E. Macias-Sanchez, N. Sommerdijk, A. Akiva

This study demonstrates that collagen mineralization in bone is driven by the extracellular matrix, not cellular activity, using a 3D cell-free bone model.

bioRxiv · Oct 24, 2022Biochemistry

Precise targeting for 3D cryo-correlative light and electron microscopy volume imaging of tissues using a FinderTOP

M. de Beer, D. Daviran, R. Roverts, L. Rutten, e. M. Sanchez, J. Metz, n. Sommerdijk, A. Akiva

This paper introduces a method for 3D cryo-correlative light and electron microscopy of tissues using a FinderTOP pattern for precise targeting.

bioRxiv · Jul 31, 2022Cell Biology

Bioinspired Silk Fibroin Mineralization for Advanced In Vitro Bone Remodeling Models

B. W. M. de Wildt, R. van der Meijden, P. A. A. Bartels, N. A. J. M. Sommerdijk, A. Akiva, K. Ito, S. Hofmann

This study develops a bioinspired silk fibroin-based scaffold for in vitro bone remodeling that supports osteoclast and osteoblast interactions, reducing animal experiments and advancing drug testing for osteoporosis.

bioRxiv · Jun 17, 2022Bioengineering

DNA uptake by cell wall-deficient bacteria reveals a putative ancient macromolecule uptake mechanism

R. Kapteijn, S. Shitut, D. Ashmann, L. Zhang, M. de Beer, D. Daviran, R. Roverts, A. Akiva, G. van Wezel, A. Kros, D. Claessen

The paper identifies a DNA uptake mechanism in cell wall-deficient bacteria that resembles eukaryotic endocytosis and may represent an ancient process for macromolecule acquisition.

bioRxiv · Jan 27, 2022Microbiology

HPM Live μ for full CLEM workflow

X. Heiligenstein, M. de Beer, J. Heiligenstein, F. Eyraud, L. Manet, F. Schmitt, E. Lamers, J. Lindenau, M. Kea-te Lindert, J. Salamero, G. Raposo, N. sommerdijk, M. Belle, A. Akiva

This paper introduces a high-pressure freezing system for cryo-fixation and describes its integration with light microscopy for correlative light and electron microscopy workflows.

bioRxiv · Sep 3, 2020 · PreprintBiophysics

An Organoid for woven Bone

A. Akiva, J. Melke, S. Ansari, N. Liv, R. van der Meijden, M. van Erp, F. Zhao, M. Stout, W. H. Nijhuis, C. de Heus, C. Muniz Ortera, J. Fermie, J. Klumperman, K. Ito, N. Sommerdijk, S. Hofmann

This study develops a 3D in vitro organoid model of woven bone formation using human bone marrow stromal cells, demonstrating osteoblast and osteocyte co-culture and mineralization.

bioRxiv · Jun 15, 2020Bioengineering