G. Raposo

Extracellular vesicles released by keratinocytes regulate melanosome maturation, melanocyte dendricity and pigment transfer

This study shows keratinocyte-derived extracellular vesicles regulate melanocyte dendrite formation, melanosome maturation, and pigment transfer, contributing to skin pigmentation and photoprotection.

Biogenesis of specialized lysosomes in differentiated keratinocytes relies on close apposition with the Golgi apparatus

This study identifies a novel mechanism where Golgi-lysosome contact in differentiated keratinocytes supports specialized lysosome function and protein secretion.

Human Cytomegalovirus modifies placental small extracellular vesicle secretion and composition towards a proviral phenotype to enhance infection of fetal recipient cells

This study shows hCMV infection alters placental sEVs to promote viral spread to fetal cells.

PI4P and BLOC-1 remodel endosomal membranes into tubules

This study shows how PI4P and BLOC-1 coordinate to form and stabilize endosomal tubules, essential for membrane remodeling and pathogen trafficking.

Two type II phosphatidylinositol 4-kinases function sequentially in tubule-mediated cargo delivery from early endosomes to melanosomes

The paper shows that PI4KII and PI4KIIβ sequentially generate PtdIns4P to support BLOC-1-dependent tubule elongation for melanosome content delivery.

Human cytomegalovirus infection changes the pattern of surface markers of small extracellular vesicles isolated from first trimester placental histocultures

This study examines how human cytomegalovirus infection alters surface markers on placental-derived small extracellular vesicles from first trimester histocultures.

Catabolism of lysosome-related organelles in color-changing spiders supports intracellular turnover of pigments

This study examines pigment organelle catabolism in crab spiders, showing they belong to the LRO family and undergo intracellular degradation without cell death or secretion.

Dynlt3, a novel fundamental regulator of melanosome movement, distribution, maturation and transfer

This study identifies Dynlt3 as a critical regulator of melanosome transport, maturation, and transfer in melanocytes, revealing its role in skin pigmentation through interactions with the Wnt/β-catenin pathway.

Microvilli-derived Extracellular Vesicles Govern Morphogenesis in Drosophila wing epithelium

The paper identifies microvilli as the source of extracellular vesicles that transport Hedgehog morphogen in Drosophila wing discs, revealing the role of Prominin-like protein and cytoskeletal components in this process.

HPM Live μ for full CLEM workflow

This paper introduces a high-pressure freezing system for cryo-fixation and describes its integration with light microscopy for correlative light and electron microscopy workflows.

Centrosome amplification mediates small extracellular vesicles secretion via lysosome disruption

The paper shows centrosome amplification in pancreatic cancer cells increases small extracellular vesicle secretion through lysosomal dysfunction, promoting tumor invasion.

Caveolae coupling of melanocytes signaling and mechanics is required for human skin pigmentation

This study shows that caveolae in melanocytes couple signaling from keratinocytes to mechanical changes, essential for skin pigmentation and tissue homeostasis.

Live tracking of inter-organ communication by endogenous exosomes in vivo

This paper develops a zebrafish model to track endogenous exosomes in vivo, revealing their release, uptake, and trophic role in inter-organ communication.