J. Klumperman

A nonenzymatic dependency on inositol-requiring enzyme 1 controls cancer cell cycle progression and tumor growth

This study identifies a nonenzymatic role of IRE1 in cancer cell cycle progression and tumor growth, independent of its kinase-RNase activity.

A novel antifolate suppresses growth of FPGS-deficient cells and overcomes methotrexate resistance

This study identifies a novel antifolate, C1, that overcomes methotrexate resistance by targeting FPGS-deficient cells through DHFR inhibition.

Impaired LAIR-1-mediated immune control due to collagen degradation in fibrosis

The paper investigates how impaired LAIR-1-collagen interaction in fibrosis contributes to inflammation and fibrosis, finding that dysfunctional collagen ligands and matrix metalloproteinases drive disease progression.

ER - lysosome contacts at a pre-axonal region regulate axonal lysosome availability

The paper shows that ER tubules regulate lysosome transport into axons by promoting lysosome homo-fission and fission-driven translocation, with ER-lysosome contacts at a pre-axonal region facilitating this process.

Morpho-functional characterization of the endo-lysosomal system by high-throughput correlative light-electron microscopy

The paper uses correlative light-electron microscopy to characterize the ultrastructural distribution of endosomal proteins at endogenous levels.

Bimodal Endocytic Probe for Three-Dimensional Correlative Light and Electron Microscopy

This paper introduces fBSA-Au, a bimodal endocytic tracer for 2D and 3D CLEM, enabling accurate registration of fluorescence to EM data with sub-200 nm precision.

Correlative Organelle Microscopy: fluorescence guided volume electron microscopy of intracellular processes

This paper introduces a targeted volume-CLEM pipeline that enables high-precision 3D correlation between fluorescence and electron microscopy data, enhancing throughput and enabling study of rare cellular events.

The stress-sensing domain of activated IRE1α forms helical filaments in narrow ER membrane tubes

This study determines the in-situ structure of IRE1 oligomers in ER tubes using cryo-CLEM, revealing helical filaments formed by IRE1 lumenal domain dimers and suggesting positive feedback in IRE1 signaling.

Aquaporin-3 regulates endosome-to-cytosol transfer via lipid peroxidation for cross presentation

This study identifies aquaporin-3 as a regulator of endosome-to-cytosol transfer by facilitating hydrogen peroxide entry, which induces lipid peroxidation and antigen release.

An Organoid for woven Bone

This study develops a 3D in vitro organoid model of woven bone formation using human bone marrow stromal cells, demonstrating osteoblast and osteocyte co-culture and mineralization.

Pancreatic beta-cell specific deletion of VPS41 causes diabetes due to defects in insulin secretion

This study shows that VPS41 deletion in pancreatic beta-cells causes diabetes by impairing insulin secretory granule formation and secretion.

Cysteamine-bicalutamide combination treatment restores alpha-ketoglutarate and corrects proximal tubule phenotype in cystinosis

This study identifies alpha-ketoglutarate as a key metabolite in cystinosis and shows that combining cysteamine with bicalutamide corrects proximal tubule defects in cell models and zebrafish.

A paralog-specific role of the COPI pathway in the neuronal differentiation of murine pluripotent cells

This study identifies a specific role for γ1-COP in neurite outgrowth during neuronal differentiation of mouse pluripotent cells, while showing functional redundancy between γ1-COP and γ2-COP.

VPS41 recessive mutation causes ataxia and dystonia with retinal dystrophy and mental retardation by inhibiting HOPS function and mTORC1 signaling

This paper identifies VPS41 mutations causing neurodegenerative disease by disrupting HOPS function and mTORC1 signaling, leading to lysosomal dysfunction and impaired autophagy.

A metabolic switch from OXPHOS to glycolysis is essential for cardiomyocyte proliferation in the regenerating heart

This study identifies a metabolic switch from OXPHOS to glycolysis in regenerating zebrafish hearts, showing it is essential for cardiomyocyte proliferation and heart regeneration.

Dynamic Kinetochore Size Regulation Promotes Microtubule Capture And Chromosome Biorientation In Mitosis

The paper shows that Spindly-based dynamic regulation of kinetochore size ensures proper microtubule capture and chromosome segregation by controlling corona expansion and shedding.