Author

A. S. Gladfelter

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Cell Biology· 8 Biophysics· 4

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 7 Research Focus:cell type annotation· 6 Research Focus:cell signaling & trafficking· 5 Research Focus:chromatin & epigenetic regulation· 4 Assay:proteomics· 4 Approach:statistical model· 4 Organism:fungi (other)· 3 Assay:imaging· 2

Frequent Co-Authors

I. Seim· 4 shared K. S. Cannon· 4 shared B. L. Woods· 3 shared A. E. Posey· 2 shared B. M. Stormo· 2 shared G. McLaughlin· 2 shared J. M. Crutchley· 2 shared R. V. Pappu· 2 shared

Activity by Year

2017-2023 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 19 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

4 shared papersBiophysics

I. Seim

Mutual co-authors in cluster

Author profile →

12 papers

Recent·Most cited

Biomolecular condensates in fungi are tuned to function at specific temperatures

B. M. Stormo, G. McLaughlin, L. Frederick, A. Jalihal, S. Cole, I. Seim, F. Dietrich, A. S. Gladfelter

The paper shows that temperature affects the material properties of Whi3 condensates in fungi, which are optimized by sequence changes for specific climates.

bioRxiv · Nov 27, 2023Cell Biology

Dynamical control enables the formation of demixed biomolecular condensates

A. Z. Lin, K. M. Ruff, A. P. Jalihal, F. Dar, M. R. King, J. M. Lalmansingh, A. E. Posey, I. Seim, A. S. Gladfelter, R. V. Pappu

This study identifies dynamical control as a mechanism enabling the formation of demixed biomolecular condensates by showing that asynchronous molecular production is required for their coexistence.

bioRxiv · Jan 4, 2023Biophysics

A kinetic basis for curvature sensing by septins

W. Shi, K. S. Cannon, B. N. Curtis, C. Edelmaier, A. S. Gladfelter, E. Nazockdast

This study shows septin curvature sensing is kinetically determined, influenced by bulk concentration and available membrane curvatures, through cooperative assembly of septin oligomers.

bioRxiv · May 16, 2022 · PreprintBiophysics

Quantitative single molecule RNA-FISH and RNase-free cell wall digestion in Neurospora crassa.

B. Bartholomai, A. S. Gladfelter, J. J. Loros, J. C. Dunlap

The paper develops a method for smFISH in Neurospora crassa by introducing a chitinase for RNase-free cell wall digestion and providing image analysis tools.

bioRxiv · Jul 19, 2021Cell Biology

Membrane surfaces regulate assembly of a ribonucleoprotein condensate

W. T. Snead, T. M. Gerbich, I. Seim, Z. Hu, A. S. Gladfelter

The paper shows membrane surfaces control the size and position of ribonucleoprotein condensates by altering diffusion rates of proteins and nucleic acids.

bioRxiv · Apr 24, 2021 · PreprintBiophysics

Dilute phase oligomerization can oppose phase separation and modulate material properties of a ribonucleoprotein condensate

I. Seim, A. E. Posey, W. T. Snead, B. M. Stormo, D. Klotsa, R. V. Pappu, A. S. Gladfelter

This study shows that a specific sequence motif in the Whi3 protein promotes dilute phase oligomerization, which opposes phase separation and alters the composition and material properties of ribonucleoprotein condensates.

bioRxiv · Apr 19, 2021Biophysics

Biophysical properties governing septin assembly

B. L. Woods, I. L. Seim, J. Liu, G. McLaughlin, K. S. Cannon, A. S. Gladfelter

This study shows septin filaments are isodesmic polymers, and regulatory proteins modulate their biophysical properties to form different structures in yeast.

bioRxiv · Mar 22, 2021 · PreprintCell Biology

Interplay of septin amphipathic helices in sensing membrane-curvature and filament bundling

B. L. Woods, K. S. Cannon, A. S. Gladfelter

This study investigates the role of amphipathic helices in septin proteins, showing their involvement in membrane curvature sensing and filament bundling.

bioRxiv · May 12, 2020Cell Biology

3D neural network-based particle tracking reveals spatial heterogeneity of the cytosol

G. A. McLaughlin, E. M. Langdon, J. M. Crutchley, L. J. Holt, M. G. Forest, J. M. Newby, A. S. Gladfelter

The paper develops a 3D neural network-based pipeline to track cytosolic nanoparticles in fungi, revealing spatial heterogeneity in diffusivity within and between cells.

bioRxiv · Oct 30, 2019 · PreprintCell Biology

Regulation of FXR1 by alternative splicing is required for muscle development and controls liquid-like condensates in muscle cells

J. A. Smith, E. G. Curry, R. E. Blue, C. Roden, S. E. R. Dundon, A. Rodriguez-Vargas, D. C. Jordan, X. Chen, S. M. Lyons, J. Crutchley, P. Anderson, M. E. Horb, A. S. Gladfelter, J. Giudice

This study shows that alternative splicing of FXR1 regulates its function in muscle development by controlling RNA-dependent condensates and disease-related mis-splicing.

bioRxiv · Oct 24, 2019Cell Biology

An amphipathic helix enables septins to sense micron-scale membrane curvature

K. S. Cannon, B. L. Woods, J. M. Crutchley, A. S. Gladfelter

This study identifies an amphipathic helix in septins that enables curvature sensing at sub-micrometer scales, independent of filament length.

bioRxiv · Nov 17, 2018Cell Biology

LITE microscopy: a technique for high numerical aperture, low photobleaching fluorescence imaging

T. C. Fadero, T. M. Gerbich, K. Rana, A. Suzuki, M. DiSalvo, K. N. Schaefer, J. K. Heppert, T. C. Boothby, B. Goldstein, M. Peifer, N. L. Allbritton, A. S. Gladfelter, A. S. Maddox, P. S. Maddox

The paper introduces LITE microscopy, a technique that improves resolution and brightness while reducing photodamage in fluorescence imaging.

bioRxiv · Oct 4, 2017Cell Biology