Time-Resolved Crystallography Reveals the Mechanisms of GTP hydrolysis for N-RAS and the Oncogenic Mutants G12C, G12V and Q61L
Guowu Lin, Paola Zinser Peniche, Xiaohong Zhou, Ulises Santiago, Igor V. Kurnikov, Silvia Russi, Brisa Caroline Alves Chagas, M.E. Sharpe, Dennis P. Stegmann, Shannon A. Heinig, Rohan K. Goyal, Veerappan Nagarajan, Daniel Calero, Sandra Viviana Vergara, Alexander Deiters, , Guillermo A. Calero
This study uses time-resolved crystallography to capture GTP hydrolysis intermediates of N-RAS and oncogenic mutants, revealing shared and distinct reaction mechanisms.
bioRxiv · Aug 21, 2025 · PreprintCancer Biology