Author

E. M. Pujadas

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 1 Biophysics· 1 Cell Biology· 1

Signature Assays, Organisms & Methods

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Research Focus:chromatin & epigenetic regulation· 3 Approach:experimental assay· 3 Organism:human· 2 Assay:imaging· 2 Research Focus:cell signaling & trafficking· 1 Research Focus:cell type annotation· 1 Assay:Hi-C· 1 Approach:microscopy & imaging analysis· 1

Frequent Co-Authors

V. Backman· 3 shared L. Almassalha· 2 shared N. Acosta· 2 shared A. A. McCarthy· 1 shared A. D. Stephens· 1 shared A. Daneshkhah· 1 shared A. Janssen· 1 shared A. Kuklinski· 1 shared

Activity by Year

2023-2024 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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3 shared papersBiophysics

V. Backman

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Author profile →

3 papers

Recent·Most cited

Formamide denaturation of double-stranded DNA for fluorescence in situ hybridization (FISH) distorts nanoscale chromatin structure

A. R. Shim, J. Frederick, E. M. Pujadas, T. Kuo, I. C. Ye, J. A. Pritchard, C. L. Dunton, P. Carrillo Gonzalez, N. Acosta, S. Jain, N. M. Anthony, L. M. Almassalha, I. Szleifer, V. Backman

This study shows that formamide in 3D FISH distorts nanoscale chromatin structure, while alternative methods like RASER-FISH and CRISPR-Sirius have minimal impact.

bioRxiv · Mar 1, 2024Biophysics

DNA density is a better indicator of a nuclear bleb than lamin B loss

S. Bunner, K. Prince, K. Srikrishna, E. M. Pujadas, P. Pellegrino, J. Aiello, A. A. McCarthy, C. Lawlor, S. Jagtap, A. Kuklinski, G. Yas, N. LaPointe, I. Eweka, E. Eastin, O. Jackson, , A. D. Stephens

The paper compares lamin B and DNA levels in nuclear blebs across cell types and perturbations, finding DNA concentration is more consistently reduced than lamin B levels.

bioRxiv · Feb 6, 2024 · PreprintCell Biology

Depletion of lamins B1 and B2 alters chromatin mobility and induces differential gene expression by a mesoscale-motion dependent mechanism

E. M. Pujadas, X. Wei, N. Acosta, L. Carter, J. Yang, L. Almassalha, A. Daneshkhah, S. S. P. Rao, V. Agrawal, F. Seker-Polat, E. L. Aiden, M. T. Kanemaki, V. Backman, M. Adli

This study shows that depleting lamins B1 and B2 changes chromatin mobility and gene expression through mesoscale-motion dependent mechanisms.

bioRxiv · Jun 26, 2023 · PreprintBioengineering