A multiplexed, target-based phenotypic screening platform using CRISPR interference in Mycobacterium abscessus
Donavan Marcus Neo, Ishay Ben-Zion, Josephine Bagnall, Matthew Y. Solomon, Austin N. Bond, Emily Gath, Shuting Zhang, Noam Shoresh F, James E. Gomez, Deborah Tan Hung
This paper introduces a CRISPRi-based PROSPECT platform for drug discovery in M. abscessus, identifying new compound targets and revealing unexpected susceptibility to isoniazid.
ACS Infectious Diseases · Oct 27, 2025Peer-reviewedMicrobiology