Josephine Bagnall

This paper introduces a CRISPRi-based PROSPECT platform for drug discovery in M. abscessus, identifying new compound targets and revealing unexpected susceptibility to isoniazid.

The paper shows that Kmo produces 3-HK, which promotes macrophage lysosomal acidification via kainate receptor antagonism, enhancing resistance to Salmonella infection.

The paper identifies a Pseudomonas aeruginosa-specific small molecule, BRD1401, that targets OprH and disrupts its interaction with LPS, increasing membrane fluidity.

This paper introduces PerSpecTM, a method to predict antibiotic mechanisms of action by comparing transcriptional responses of new molecules to known perturbations, demonstrating its effectiveness with three new anti-Pseudomonas aeruginosa compounds.