Precision super-resolution cryo-correlative light and electron microscopy for rapid in situ structural analyses of optogenetically-positioned organelles
G. M. I. Redpath, J. A. Rae, Y. Yao, J. Ruan, M. L. Cagigas, R. Whan, E. C. Hardeman, P. W. Gunning, V. Ananthanarayanan, R. G. Parton, N. Ariotti
The paper develops a cryo-CLEM workflow using optogenetics to enable rapid in situ structural analysis of organelles without cryo-focused ion beam milling.
bioRxiv · Nov 22, 2022Cell Biology