R. G. Parton

Flexing fish: cell surface plasticity in response to shape change in the whole organism

The paper develops an active trapping model to study caveolae's role in maintaining sarcolemmal integrity during shape changes in zebrafish.

Caveola mechanotransduction reinforces the cortical cytoskeleton to promote epithelial resistance

The paper shows that caveola disassembly under mechanical stress reinforces the actin cortex at adherens junctions, enhancing epithelial resistance to tensile stress.

Precision super-resolution cryo-correlative light and electron microscopy for rapid in situ structural analyses of optogenetically-positioned organelles

The paper develops a cryo-CLEM workflow using optogenetics to enable rapid in situ structural analysis of organelles without cryo-focused ion beam milling.

FBXL4 suppresses mitophagy by restricting the accumulation of NIX and BNIP3 mitophagy receptors

This study identifies FBXL4 as a suppressor of basal mitophagy by ubiquitylating and degrading NIX and BNIP3, and shows pathogenic variants disrupt this function leading to mtDNA depletion syndrome.

A role for caveolar proteins in regulation of the circadian clock

This study shows that cavin proteins modulate the circadian clock by interacting with PER2 and responding to stimuli like oxidative stress and calcium influx.

Dynamin2 stabilizes plasma membrane-connected caveolae by restraining fission

This study uses live-cell microscopy to show that Dyn2 stabilizes caveolae by preventing their fission, independent of its GTPase activity or EHD2.

Sugar transporter Slc37a2 regulates bone metabolism via a dynamic tubular lysosomal network in osteoclasts

This study identifies Slc37a2 as a sugar transporter in osteoclast secretory lysosomes, showing its role in bone metabolism through dynamic tubular networks and its impact on bone mass.

Proteostatic regulation of caveolins avoids premature oligomerisation and preserves ER homeostasis

This study investigates proteostatic regulation of caveolin-1 and caveolin-3, showing how their trafficking and oligomerisation are controlled to prevent ER stress and disease.

A mechanosensing mechanism mediated by IRSp53 controls plasma membrane shape homeostasis at the nanoscale

The paper identifies a mechanosensing mechanism involving IRSp53 that detects and restores plasma membrane nano-scale deformations through actin polymerization.

Caveolae respond to acute oxidative stress through membrane lipid peroxidation, cytosolic release of CAVIN1, and downstream regulation of NRF2

This study shows that caveolae regulate oxidative stress response by lipid peroxidation, CAVIN1 release, and NRF2 interaction, affecting cell death and regeneration.

YhcB coordinates peptidoglycan and LPS biogenesis with phospholipid synthesis during Escherichia coli cell growth.

This study identifies YhcB as a coordinator of peptidoglycan, LPS, and phospholipid synthesis in E. coli, revealing its role in envelope stability and cell size control.

Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation

This study shows that Cavin1's intrinsically disordered domains are essential for caveola formation and trafficking through electrostatic interactions and phase separation.

De novo macrocyclic peptides for inhibiting, stabilising and probing the function of the Retromer endosomal trafficking complex

The paper develops macrocyclic peptides that inhibit, stabilize, and probe the Retromer complex's function, showing high affinity and specificity for different subunits.

In vivo proteomic mapping through GFP-directed proximity dependent biotin labelling in zebrafish

The paper introduces a method to map protein interactions in zebrafish using GFP-directed biotin labelling and applies it to study caveolin interactors in muscle cells.

Nanoscape, a data-driven 3D real-time interactive virtual cell environment

Nanoscape provides a 3D interactive virtual cell environment using scientific data and artistic design for real-time exploration of cellular structures.

APEX-Gold: A genetically-encoded particulate marker for robust 3D electron microscopy

APEX-Gold converts APEX reaction products into silver/gold particles for improved EM detection of tagged proteins.

Cargo-specific recruitment in clathrin and dynamin-independent endocytosis

The paper identifies swiprosin-1 as a cargo-specific adaptor for the CG-pathway, showing its role in integrin internalization and breast cancer progression.

Cavin3 released from caveolae interacts with BRCA1to regulate the cellular stress response

This study shows cavin3 interacts with BRCA1 to regulate DNA repair and cellular stress response, with implications for cancer.

An inverted Caveolin-1 topology defines a novel exosome secreted from prostate cancer cells

The paper identifies an inverted Cav1 topology in exosomes secreted by prostate cancer cells, revealing a non-canonical autophagy pathway involved in disease progression.

An Endocytic Capture Model for Skeletal Muscle T-tubule Formation

This study develops a four-dimensional model of T-tubule formation in zebrafish using live imaging and electron microscopy, proposing an endocytic capture mechanism involving dynamic endocytic tubules, myofibrils, and membrane delivery from recycling endosomes and Golgi.

Dissecting the nanoscale lipid profile of caveolae

This study defines the caveolar lipid profile using quantitative lipid mapping and simulations, showing how CAV1 and cavin1 sort distinct lipids and create a unique lipid environment.

Piezo1 Induces Local Curvature in a Mammalian Membrane and Forms Specific Protein-Lipid Interactions

This study shows Piezo1 induces membrane curvature and forms specific lipid interactions, influencing both membrane morphology and lipid composition.

Caveolae set levels of epithelial monolayer tension to eliminate tumor cells

The paper shows caveolae regulate epithelial tension through lipid signaling, enabling the elimination of oncogene-transformed cells by apical extrusion.

Drug-induced increase in lysobisphosphatidic acid reduces the cholesterol overload in Niemann-Pick type C cells and mice

The paper identifies thioperamide as a drug that increases lysobisphosphatidic acid (LBPA) levels, reducing cholesterol overload in Niemann-Pick type C cells and mice.

Mechanochemical feedback and control of endocytosis and membrane tension

The paper identifies a negative feedback loop between membrane tension and the CLIC/GEEC pathway mediated by vinculin and GBF1.

Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria

The paper identifies a novel pathway involving Rab5 and Alsin that regulates stress-activated cytoprotective signaling on mitochondria, linking mitochondrial stress response to ALS pathology.

Small GTPases and BAR domain proteins regulate branched actin to make clathrin and dynamin independent endocytic vesicles

This study identifies the molecular machinery regulating clathrin and dynamin-independent endocytic vesicles, showing the sequential recruitment of ARF1/GBF1 and CDC42, and the roles of BAR domain proteins and actin polymerization.