N. Ariotti

Molecular basis for the assembly of the Vps5-Vps17 SNX-BAR proteins with Retromer

This study investigates how Vps5 and Vps17 SNX-BAR proteins assemble with Retromer, revealing a PL motif in Vps5 that binds Vps29 and is essential for membrane tubule association and cargo recycling.

Flexing fish: cell surface plasticity in response to shape change in the whole organism

The paper develops an active trapping model to study caveolae's role in maintaining sarcolemmal integrity during shape changes in zebrafish.

Karyopherin mimicry explains how the HIV capsid penetrates nuclear pores

This paper shows how the HIV capsid mimics karyopherins to penetrate nuclear pores by interacting with FG-motifs, enabling it to bypass the size limit of the nuclear pore complex.

Precision super-resolution cryo-correlative light and electron microscopy for rapid in situ structural analyses of optogenetically-positioned organelles

The paper develops a cryo-CLEM workflow using optogenetics to enable rapid in situ structural analysis of organelles without cryo-focused ion beam milling.

Microtubule-mitochondrial attachmentfacilitates cell division symmetry and propermitochondrial partitioning in fission yeast

The paper shows that microtubule-mitochondrial attachment in fission yeast ensures symmetric cell division and proper mitochondrial distribution.

Single-molecule imaging of cytoplasmic dynein in cellulo reveals the mechanism of motor activation and cargo movement

This study uses single-molecule imaging to reveal how dynein activation and cargo movement occur through stochastic interactions and dynamic binding kinetics.

Muscle-specific Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle

This study identifies Cavin4's role in T-tubule formation and stability in skeletal muscle by showing its interaction with Bin1 and effects on caveolae remodeling.

APEX-Gold: A genetically-encoded particulate marker for robust 3D electron microscopy

APEX-Gold converts APEX reaction products into silver/gold particles for improved EM detection of tagged proteins.

Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation

This study shows that Cavin1's intrinsically disordered domains are essential for caveola formation and trafficking through electrostatic interactions and phase separation.

An inverted Caveolin-1 topology defines a novel exosome secreted from prostate cancer cells

The paper identifies an inverted Cav1 topology in exosomes secreted by prostate cancer cells, revealing a non-canonical autophagy pathway involved in disease progression.

An Endocytic Capture Model for Skeletal Muscle T-tubule Formation

This study develops a four-dimensional model of T-tubule formation in zebrafish using live imaging and electron microscopy, proposing an endocytic capture mechanism involving dynamic endocytic tubules, myofibrils, and membrane delivery from recycling endosomes and Golgi.

Dissecting the nanoscale lipid profile of caveolae

This study defines the caveolar lipid profile using quantitative lipid mapping and simulations, showing how CAV1 and cavin1 sort distinct lipids and create a unique lipid environment.