G. Pegoraro

Allele-level visualization of transcription and chromatin by high-throughput imaging

The paper develops high-throughput DNA/RNA FISH methods to visualize allele-level chromatin features and gene activity across cell populations.

A nuclear architecture screen in Drosophila identifies Stonewall as a link between chromatin position at the nuclear periphery and germline stem cell fate

The paper identifies Stonewall as a protein that positions chromatin at the nuclear periphery in Drosophila, linking this positioning to gene repression and germline stem cell fate.

Identification of epigenetic modulators as determinants of nuclear size and shape

This study identifies epigenetic modulators that influence nuclear size and shape through a high-throughput siRNA screen and biochemical analysis.

HiFENS: High-throughput FISH detection of endogenous pre-mRNA splicing isoforms

HiFENS is a high-throughput imaging assay that detects endogenous splicing isoforms, used to identify upstream regulators of alternative splicing in human cells.

HiIDDD: A high-throughput imaging pipeline for the quantitative detection of DNA damage in primary human immune cells

HiIDDD provides a high-throughput imaging method to detect DNA damage in primary human immune cells, showing consistent results across cell types and donors.

Activation of endoplasmic reticulum stress via clustering of inner nuclear membrane proteins

The paper identifies nuclear protein aggregation as a trigger for endoplasmic reticulum stress in Hutchinson-Gilford Progeria Syndrome.

A Deep Learning Pipeline for Nucleus Segmentation

This paper develops a deep learning pipeline to evaluate nuclear segmentation models trained on small, custom annotated datasets, showing they can match or exceed performance of pre-trained models.

IRAK1-mediated coincidence detection of microbial signals licenses inflammasome activation

This study identifies IRAK1-mediated supramolecular organizing centers that coordinate multiple immune pathways to license inflammasome activation in response to microbial signals.

The nucleoporin ELYS regulates nuclear size by controlling NPC number and nuclear import capacity

This study identifies ELYS as a regulator of nuclear size by controlling NPC number and nuclear import capacity in mammalian cells.

Effects of Human Sex Chromosome Dosage on Spatial Chromosome Organization

This study investigates how variations in sex chromosome dosage affect chromosome organization in primary cells from SCA patients using HiCTMap imaging.

HiCTMap: Detection and Analysis of Chromosome Territory Structure and Position by High-throughput Imaging

HiCTMap enables high-throughput imaging and analysis of chromosome territories in mammalian cells, providing precise 2D and 3D positioning and quantification.