H. Ausserwoger

Temperature-induced changes in protein interactions control RNA recruitment to G3BP1 condensates

The paper investigates how temperature affects G3BP1-RNA phase separation, revealing that while the phase boundary remains stable, stoichiometry and energetics shift, driven by entropic processes.

Linking modulation of bio-molecular phase behaviour with collective interactions

This paper introduces a dominance analysis framework to determine the molecular mechanism of condensate modulation, demonstrating its application to G3BP1/RNA condensates disrupted by suramin.

Glutamate helps unmask the differences in driving forces for phase separation versus clustering of FET family proteins in sub-saturated solutions

The paper shows that glutamate reveals distinct energy and length scales for phase separation and clustering of FET family proteins in sub-saturated solutions.

Dominance Analysis: A formalism to uncover dominant energetic contributions to biomolecular condensate formation in multicomponent systems

This paper introduces a formalism to identify dominant energetic contributions to biomolecular condensate formation in multicomponent systems by analyzing phase separation data.

Modulating Nucleic Acid Phase Transitions as a Mechanism of Action for Cell-Penetrating Antimicrobial Peptides

This study identifies that antimicrobial peptides (AMPs) induce nucleic acid phase separation, which may be a novel mechanism for their antibacterial activity.

Adsorption of RNA to interfaces of biomolecular condensates enables wetting transitions

This study shows that pyrimidine-rich RNAs act as adsorbents enabling wetting transitions in condensates formed by purine-rich RNAs, revealing orientation differences between scaffolds and adsorbents.

Multi-dimensional protein solubility optimization with an ultra-high-throughput microfluidic platform

This paper introduces a high-throughput microfluidic platform for protein solubility screening, enabling rapid and efficient optimization of formulation additives and mutations.

Multiphase condensates from a kinetically arrested phase transition

This paper shows that multiphase biomolecular condensates form due to kinetic trapping during phase transitions, not thermodynamic stability, and explains how this leads to internal sub-compartments.