A. A. Hyman

Actin polymerization counteracts prewetting of N-WASP on supported lipid bilayers

The paper shows that actin polymerization counteracts N-WASP prewetting on lipid bilayers, revealing a balance between condensate formation and dissolution in cortical condensates.

Intra-condensate demixing of TDP-43 inside stress granules generates pathological aggregates

This study shows that TDP-43 aggregation in stress granules requires up-concentration and oxidative stress, leading to intra-condensate demixing and pathological aggregates.

Small molecule modulation of a redox-sensitive stress granule protein dissolves stress granules with beneficial outcomes for familial amyotrophic lateral sclerosis models

The paper identifies lipoamide as a small molecule that dissolves stress granules and improves outcomes in ALS models by modulating redox-sensitive proteins.

Glutamate helps unmask the differences in driving forces for phase separation versus clustering of FET family proteins in sub-saturated solutions

The paper shows that glutamate reveals distinct energy and length scales for phase separation and clustering of FET family proteins in sub-saturated solutions.

Assembly of tight junction belts by surface condensation and actin elongation

The paper investigates how surface phase transitions control protein condensation to form tight junction belts through ZO-scaffold proteins and actin polymerization.

Quantifying collective interactions in biomolecular phase separation

This study quantifies collective interactions in biomolecular phase separation by analyzing dilute phase concentrations and free energy, revealing how salts and small molecules modulate condensate formation.

A conserved and tunable mechanism for the temperature-controlled condensation of the translation factor Ded1p

The paper investigates how Ded1p translation factors assemble into condensates in response to heat across fungi, identifying structural and amino acid determinants of temperature sensitivity.

SAMHD1 controls innate immunity by regulating condensation of immunogenic self RNA

This study shows SAMHD1 regulates innate immunity by controlling RNA condensation, preventing immune recognition of self RNA and reducing inflammation.

Phase separating RNA binding proteins form heterogeneous distributions of clusters in subsaturated solutions

This study investigates cluster formation in subsaturated solutions of RNA binding proteins with prion-like domains and finds that cluster formation and phase separation are coupled through sequence-specific interactions.

Molecular mechanisms of stress-induced reactivation in mumps virus condensates

The paper investigates how cellular stress triggers reactivation of persistent mumps virus infection through molecular and structural changes in viral condensates.

HspB8 prevents aberrant phase transitions of FUS by chaperoning its folded RNA binding domain

This study identifies HspB8's role in preventing FUS condensate hardening by interacting with its RNA-binding domain, suggesting a mechanism to prevent neurodegenerative disease-associated phase transitions.

Quantitative Theory for the Diffusive Dynamics of Liquid Condensates

The paper develops a physics-based theory for quantifying diffusive dynamics in liquid condensates and validates it using fluorescence recovery after photobleaching experiments.

Reentrant liquid condensate phase of proteins is stabilized by hydrophobic and non-ionic interactions

The paper shows that proteins forming liquid condensates can reenter a phase-separated state at high salt concentrations due to hydrophobic and non-ionic interactions, distinct from low-salt electrostatic-driven phase separation.

Local thermodynamics governs the formation and dissolution of protein condensates in living cells

The paper investigates how temperature changes affect P granule condensates in C. elegans, showing their reversible dissolution and recondensation, which can be modeled by thermodynamic equilibrium principles.

Label-free composition determination for biomolecular condensates with an arbitrarily large number of components

This paper introduces a label-free method to measure the composition of biomolecular condensates with many components, showing it can capture complex dynamics and stoichiometry without perturbing the system.

Surface condensation of a pioneer transcription factor on DNA

This study uses optical tweezers to observe Klf4 condensation on DNA, showing surface-mediated microphases that reconcile phase separation with nuclear organization.

High Resolution Biomolecular Condensate Phase Diagrams with a Combinatorial Microdroplet Platform

This paper introduces a microdroplet platform to rapidly generate high-resolution phase diagrams of biomolecular condensates and demonstrates its application in studying phase transitions.

A localization screen reveals translation factories and widespread co-translational RNA targeting

The paper identifies translation factories and co-translational RNA targeting through a dual protein/mRNA localization screen in human cells.

The Glycolytic Protein Phosphofructokinase Dynamically Relocalizes into Subcellular Compartments with Liquid-like Properties in vivo

This study shows that PFK-1.1 dynamically forms liquid-like condensates in neurons under energy stress, revealing a new mechanism for subcellular organization.

Phase separation provides a mechanism to reduce noise in cells

The paper shows that phase separation can buffer gene expression noise by forming liquid droplets in mammalian cells, providing a fast mechanism for noise reduction.

Kinetically distinct phases of tau on microtubules regulate kinesin motors and severing enzymes

This study shows tau forms distinct kinetic phases on microtubules that regulate kinesin motors and severing enzymes like katanin.

Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria

The paper identifies a novel pathway involving Rab5 and Alsin that regulates stress-activated cytoprotective signaling on mitochondria, linking mitochondrial stress response to ALS pathology.

Phosphatase PP2A and microtubule pulling forces disassemble centrosomes during mitotic exit

The paper identifies PP2A and microtubule pulling forces as key factors in PCM disassembly during mitotic exit in C. elegans embryos.

Local Nucleation Of Microtubule Bundles Through Tubulin Concentration Into A Condensed Tau Phase

The paper shows that tau forms liquid droplets that concentrate tubulin, enabling local microtubule bundle nucleation without centrosomes.