N. A. Erkamp

Quantifying collective interactions in biomolecular phase separation

This study quantifies collective interactions in biomolecular phase separation by analyzing dilute phase concentrations and free energy, revealing how salts and small molecules modulate condensate formation.

Modulating Nucleic Acid Phase Transitions as a Mechanism of Action for Cell-Penetrating Antimicrobial Peptides

This study identifies that antimicrobial peptides (AMPs) induce nucleic acid phase separation, which may be a novel mechanism for their antibacterial activity.

Adsorption of RNA to interfaces of biomolecular condensates enables wetting transitions

This study shows that pyrimidine-rich RNAs act as adsorbents enabling wetting transitions in condensates formed by purine-rich RNAs, revealing orientation differences between scaffolds and adsorbents.

Multi-dimensional protein solubility optimization with an ultra-high-throughput microfluidic platform

This paper introduces a high-throughput microfluidic platform for protein solubility screening, enabling rapid and efficient optimization of formulation additives and mutations.

Tie-lines reveal interactions driving heteromolecular condensate formation

The paper develops a method to determine tie-line gradients in ternary LLPS systems using dilute phase concentration measurements, revealing interaction types and compositions in biomolecular condensates.

Multiphase condensates from a kinetically arrested phase transition

This paper shows that multiphase biomolecular condensates form due to kinetic trapping during phase transitions, not thermodynamic stability, and explains how this leads to internal sub-compartments.

High Resolution Biomolecular Condensate Phase Diagrams with a Combinatorial Microdroplet Platform

This paper introduces a microdroplet platform to rapidly generate high-resolution phase diagrams of biomolecular condensates and demonstrates its application in studying phase transitions.