H. Nishida

Genetic characterization of cucumber genetic resources in the NARO Genebank indicates their multiple dispersal trajectories to the East

This study uses genotyping-by-sequencing to analyze 723 cucumber accessions, revealing multiple dispersal routes to East Asia and Southeast Asia.

One-step N-terminomics based on isolation of protein N-terminal peptides from LysargiNase digests by tip-based strong cation exchange chromatography

The paper introduces a one-step N-terminomics method using tip-based SCX chromatography, enabling high-throughput and sensitive profiling of N-terminal peptides.

Centrifugal Gel Crushing for Gel-based Proteome Analysis

This paper introduces a centrifugal gel crushing method to improve protein recovery and reproducibility in gel-based proteomics by replacing manual dicing with a pipette tip-based technique.

Protein N-Terminomics Reveals Major Proteases in Regulating Beige Adipocyte Differentiation

This study identifies proteases regulating beige adipocyte differentiation through N-terminomics and functional experiments.

Classification of Extracellular Vesicles based on Surface Glycan Structures by Spongy-like Separation Media

This paper introduces a novel lectin-based affinity chromatography method using spongy monoliths to classify extracellular vesicles based on surface glycan structures and demonstrates subpopulations through proteome profiling.

One-step Isolation of Protein C-terminal Peptides from V8 Protease-digested Proteins by Metal Oxide-based Ligand Exchange Chromatography

The paper develops a one-step method to isolate protein C-terminal peptides using MOLEX chromatography, achieving high purity and coverage in human C-terminomics.