K. Imami

Proteomic analysis to classify extracellular vesicles and plasmodesmata in Arabidopsis cell culture supernatants

The paper identifies and distinguishes extracellular vesicle and plasmodesmata proteins in Arabidopsis cell culture supernatants using proteomic and imaging analyses.

One-step N-terminomics based on isolation of protein N-terminal peptides from LysargiNase digests by tip-based strong cation exchange chromatography

The paper introduces a one-step N-terminomics method using tip-based SCX chromatography, enabling high-throughput and sensitive profiling of N-terminal peptides.

Mapping adipocyte interactome networks by HaloTag enrichment mass-spectrometry

The paper introduces HaloMS, a high-throughput method for mapping protein interactions in adipocytes, identifying new transcription factor complexes and networks.

UGGT1-mediated reglucosylation of N-glycan competes with ER-associated degradation of unstable and misfolded glycoproteins

This study shows UGGT1 reglucosylation competes with EDEM-mediated degradation of misfolded glycoproteins, influencing ER protein quality control.

Liquid-liquid extraction of lipidated peptides for direct identification of lipidation sites

This paper introduces a proteomic method to identify endogenous lipidation sites using liquid-liquid extraction and mass spectrometry, finding 90 sites in HeLa cells and tissue-specific profiles in mice.

Classification of Extracellular Vesicles based on Surface Glycan Structures by Spongy-like Separation Media

This paper introduces a novel lectin-based affinity chromatography method using spongy monoliths to classify extracellular vesicles based on surface glycan structures and demonstrates subpopulations through proteome profiling.

pSNAP: Proteome-wide analysis of elongating nascent polypeptide chains

pSNAP profiles proteome-wide elongating nascent polypeptide chains, revealing translational repression and modification effects on ribosome-associated interactions.

Monitoring mitochondrial translation by pulse SILAC

This study introduces a proteomic method to quantify mitochondrial-encoded proteins and reveals post-translational regulation of OXPHOS complexes.

Exploiting a PAX3-FOXO1-induced synthetic lethal ATR dependency for rhabdomyosarcoma therapy

This study identifies ATR as a synthetic lethal target in PAX3-FOXO1-driven rhabdomyosarcoma, showing ATR inhibition enhances PARP inhibitor efficacy and is tolerated in patients.

Quantitative nascent proteome profiling by dual pulse labeling with O-propargyl-puromycin and stable isotope labeled amino acids

This paper introduces a method to accurately quantify nascent proteins by dual pulse labeling with OPP and stable isotope labeled amino acids, revealing repression of specific proteins upon transcription inhibition.

Cross-regulation of viral kinases with cyclin A secures shutoff of host DNA synthesis

The paper identifies how herpesvirus kinases interact with Cyclin A to inhibit host DNA synthesis during infection.