T. Kubo

Downregulation of Semaphorin 4A in keratinocytes reflects the features of non-lesional psoriasis

This study shows that downregulation of Sema4A in keratinocytes is associated with non-lesional psoriasis features and that Sema4A regulates skin homeostasis and inflammation.

Modifiable clinical dental impression methods to obtain whole-mouth and detailed dental traits from vertebrates

This study introduces a non-destructive method using a printable tray to capture high-resolution dental traits in vertebrates, enabling in-vivo and museum specimen analysis.

Time-deterministic cryo-optical microscopy

The paper introduces a technique to rapidly freeze biological systems during optical observation, enabling detailed time-specific analysis of cellular dynamics at high spatial resolution.

Selective plane activation structured illumination microscopy

This study improves structured illumination microscopy by using selective plane illumination and photoswitchable proteins to eliminate out-of-focus background, enabling high-resolution imaging of volumetric samples.

Analysis of antennal responses to motion stimuli in the honey bee by automated tracking using DeepLabCut

The paper uses DeepLabCut to track honey bee antennal responses to motion stimuli and applies unsupervised clustering to classify antennal movements.

Distinct roles of α- and β-tubulin C-terminal tails for ciliary function as revealed by a CRISPR/Cas9 mediated gene editing in Chlamydomonas

The paper uses CRISPR/Cas9 to generate Chlamydomonas mutants with modified tubulin C-terminal tails, revealing distinct roles in ciliary motility and assembly.

TRIAC disrupts cerebral thyroid hormone action via a negative feedback loop and heterogenous distribution among organs

This study shows TRIAC disrupts cerebral thyroid hormone action by reducing circulating TH levels and being poorly distributed to the brain, while compensating in peripheral tissues.

Classification of Extracellular Vesicles based on Surface Glycan Structures by Spongy-like Separation Media

This paper introduces a novel lectin-based affinity chromatography method using spongy monoliths to classify extracellular vesicles based on surface glycan structures and demonstrates subpopulations through proteome profiling.

Puromycin is incorporated into regenerating flagella of Chlamydomonas reinhardtii as an indicator of nascent flagellar proteins

The paper develops a non-radioactive method using puromycin to track nascent flagellar proteins in Chlamydomonas reinhardtii during regeneration.

Genetically-linked simultaneous overexpression of multiple herbicide-metabolizing genes for broad-spectrum resistance in an agricultural weed Echinochloa phyllopogon

The paper identifies genetically linked overexpression of multiple herbicide-metabolizing genes in E. phyllopogon, enhancing and broadening metabolic resistance to multiple herbicides.

Agonist and antagonist diverted twisting motions of single TRPV1 channel

The paper uses DXT to observe agonist and antagonist-induced rotational biases in TRPV1 channels, revealing opposing twisting motions and providing insights into TRP channel activation and modulation.

Wavefront-sensorless adaptive optics with a laser-free spinning disk confocal microscope

The paper introduces wavefront-sensorless adaptive optics for a laser-free spinning disk confocal microscope, improving signal, contrast, and resolution by correcting specimen-induced aberrations.