I. Koyama-Honda

The paper introduces a multi-parametric particle-based method to analyze organelle landscapes, enabling visualization of heterogeneous organelle structures and transitional states using fluorescence microscopy and UMAP.

The paper shows that STX17 recruitment to mature autophagosomes is temporally regulated by PI4P-driven changes in membrane charge.

The paper determines the average shapes of forming autophagosomes using 3D electron microscopy and develops a model showing their morphology is determined by the ratio of rim size to surface area.

This study identifies SIDT2 as a mediator of microautophagy that delivers cytosolic proteins into lysosomes, linking proteostasis to protein aggregation disease.

High-speed single-molecule imaging shows outer-leaflet rafts induce transbilayer raft phases that recruit inner-leaflet signaling molecules via lipid interactions.