N. Mizushima

Organelle landscape analysis using a multi-parametric particle-based method

The paper introduces a multi-parametric particle-based method to analyze organelle landscapes, enabling visualization of heterogeneous organelle structures and transitional states using fluorescence microscopy and UMAP.

Syntaxin 17 recruitment to mature autophagosomes is temporally regulated by PI4P accumulation

The paper shows that STX17 recruitment to mature autophagosomes is temporally regulated by PI4P-driven changes in membrane charge.

Autophagy adaptors mediate Parkin-dependent mitophagy by forming sheet-like liquid condensates

This study shows that autophagy adaptors form liquid-like condensates to facilitate mitophagy by enabling membrane contacts during Parkin-mediated mitochondrial degradation.

Comprehensive analysis of autophagic functions of WIPI family proteins and their implications for the pathogenesis of β-propeller associated neurodegeneration

This study investigates the roles of WIPI proteins in autophagy and their implications in β-propeller associated neurodegeneration, finding that WIPI2 is essential for autophagy and that some BPAN-related mutations affect autophagic activity.

Unique Amphipathic α-helix Drives Membrane Insertion and Enzymatic Activity of ATG3

The paper identifies an amphipathic α-helix in ATG3 that facilitates membrane insertion and enzymatic activity essential for autophagosome formation.

Experimental determination and mathematical modeling of standard shapes of forming autophagosomes

The paper determines the average shapes of forming autophagosomes using 3D electron microscopy and develops a model showing their morphology is determined by the ratio of rim size to surface area.

The pH-sensing Rim101 pathway regulates cell size in budding yeast

The paper identifies Rim21 as a positive regulator of cell size in budding yeast through the Rim101 pathway, showing its role in size regulation under physiological conditions and alkaline stress.

A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag

This paper introduces a HaloTag-based assay to monitor mammalian autophagic flux by detecting proteolytic processing of pulse-labeled reporters.

Phosphorylation by casein kinase 2 ensures ER-phagy receptor TEX264 binding to ATG8 proteins

The paper shows phosphorylation of TEX264's LIR by casein kinase 2 enhances its binding to ATG8 proteins, crucial for ER-phagy, through hydrogen bonds not achievable with acidic residues.

NCOA4 drives ferritin phase separation to facilitate macroferritinophagy and endosomal microferritinophagy

This study identifies NCOA4 as a driver of ferritin phase separation, enabling macroferritinophagy and endosomal microferritinophagy through multivalent interactions.

Evolution and insights into the structure and function of the DedA superfamily containing TMEM41B and VMP1

The paper identifies a new clade of bacterial proteins and reveals the evolutionary relationship and structural features of the DedA superfamily, including TMEM41B and VMP1.

Autophagy controls lipid droplet formation by fine-tuning NCoR1 levels

This study shows autophagy regulates lipid droplet formation by modulating NCoR1 levels, affecting LXR target gene expression and lipid metabolism in the liver.