Ligase-mediated programmable genomic integration (L-PGI): an efficient site-specific gene editing system that overcomes the limitations of reverse transcriptase-based editing systems
Angela X. Nan, Michael Chickering, Christopher L. Bartolome, Neeta Shadija, Dan Li, Brett Estes, Jessica R. Von Stetina, Wei Li, Jason L. Andresen, Kaivalya Molugu, Ravindra Amunugama, Minggang Fang, Chen Bai, Jie Wang, Davood Norouzi, , Jenny Xie
This paper introduces L-PGI, a ligase-mediated gene editing system that enables efficient and precise genomic integration in both dividing and nondividing cells, overcoming limitations of reverse transcriptase-based methods.
Nature Communications · Dec 10, 2025Peer-reviewedBioengineering