Jie Wang

This paper introduces L-PGI, a ligase-mediated gene editing system that enables efficient and precise genomic integration in both dividing and nondividing cells, overcoming limitations of reverse transcriptase-based methods.

The paper identifies low fidelity in RT-based genome editing in non-dividing mouse hepatocytes and proposes solutions using neonatal mice and transgenic models.

The paper develops and validates methods to detect off-target recombination by Large Serine Integrases, finding higher sensitivity than whole genome sequencing.

The paper identifies three causative genes for childhood epileptic encephalopathies through trio-based whole-exome sequencing and individualized analysis of genetic variants.