Author

Joshua P. Graham

Lehigh University

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 4

Signature Assays, Organisms & Methods

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Research Focus:cell type annotation· 3 Assay:CRISPR screen· 3 Approach:experimental assay· 3 Organism:human· 3 Research Focus:drug response / drug discovery· 2 Research Focus:gene regulatory network inference· 2 Research Focus:benchmarking· 1 Assay:bulk RNA-seq· 1

Frequent Co-Authors

Tomas Gonzalez‐FernandeziD· 4 shared Abolfazl Salehi MoghaddamiD· 1 shared Alexander Arteaga· 1 shared Anand RamamurthiiD· 1 shared Daniel J. LavertyiD· 1 shared Erika E. WheeleriD· 1 shared Helen O. McCarthyiD· 1 shared Jose Gabriel Castro· 1 shared

Activity by Year

2024-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 21 mutual co-author links

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4 shared papersBioengineering

Tomas Gonzalez‐FernandezORCID

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Author profile →

4 papers

Recent·Most cited

Spatiotemporal Control of Genetic and Epigenetic Editing through Covalent Tethering of CRISPR Nanoparticles to Zwitterionic Microgels

Joshua P. Graham, Erika E. Wheeler, Kent J. Leach, Tomas Gonzalez‐Fernandez

This paper introduces a method to covalently tether CRISPR nanoparticles to microgels for sustained and localized gene editing in human cells.

bioRxiv · Sep 22, 2026 · PreprintBioengineering

Integrative Multiomic Analysis Reveals How Non-Viral Delivery System Selection Shapes CRISPR Gene Editing Outcomes in Stem Cells.

Joshua P. Graham, Alexander Arteaga, Abolfazl Salehi Moghaddam, Kara L. Spiller, Daniel J. Laverty, Tomas Gonzalez‐Fernandez

This study compares non-viral CRISPR delivery methods in stem cells, finding that lipid-based delivery offers high efficiency but risks genomic instability, while CPPs reduce side effects at lower efficiency.

bioRxiv · May 30, 2026 · PreprintBioengineering

Versatile Cell Penetrating Peptide for Multimodal CRISPR Gene Editing in Primary Stem Cells

Joshua P. Graham, Jose Gabriel Castro, Lisette C. Werba, Joseph Amitrano, Luke C. Fardone, Kevin P. Francis, Anand Ramamurthi, Michael J. Layden, Helen O. McCarthy, Tomas Gonzalez‐Fernandez

This paper demonstrates that the RALA peptide effectively delivers CRISPR components in DNA, RNA, and RNP formats to primary mesenchymal stem cells with high viability and low cytotoxicity.

Advanced Functional Materials · Sep 2, 2025Peer-reviewedBioengineering

CRISPR-GEM: A Novel Machine Learning Model for CRISPR Genetic Target Discovery and Evaluation

Joshua P. Graham, Yu Zhang, Lifang He, Tomas Gonzalez‐Fernandez

CRISPR-GEM predicts optimal CRISPR target genes by modeling gene regulatory networks using MLPs trained on differential gene expression data.

ACS Synthetic Biology · Oct 8, 2024Peer-reviewedBioengineering