Tomas Gonzalez‐Fernandez

Spatiotemporal Control of Genetic and Epigenetic Editing through Covalent Tethering of CRISPR Nanoparticles to Zwitterionic Microgels

This paper introduces a method to covalently tether CRISPR nanoparticles to microgels for sustained and localized gene editing in human cells.

Integrative Multiomic Analysis Reveals How Non-Viral Delivery System Selection Shapes CRISPR Gene Editing Outcomes in Stem Cells.

This study compares non-viral CRISPR delivery methods in stem cells, finding that lipid-based delivery offers high efficiency but risks genomic instability, while CPPs reduce side effects at lower efficiency.

Versatile Cell Penetrating Peptide for Multimodal CRISPR Gene Editing in Primary Stem Cells

This paper demonstrates that the RALA peptide effectively delivers CRISPR components in DNA, RNA, and RNP formats to primary mesenchymal stem cells with high viability and low cytotoxicity.

Biocompatible Composite Hydrogel with On-Demand Swelling-Shrinking Properties for 4D Bioprinting

This paper develops a high-swelling composite hydrogel, SwellMA, for 4D bioprinting, showing superior swelling capacity and on-demand swelling-shrinking behavior.

CRISPR-GEM: A Novel Machine Learning Model for CRISPR Genetic Target Discovery and Evaluation

CRISPR-GEM predicts optimal CRISPR target genes by modeling gene regulatory networks using MLPs trained on differential gene expression data.