K. Mitsuoka

Mechanism of ATP hydrolysis dependent rotation of ATP synthases

The paper uses cryo-EM to capture catalytic intermediates of ATP synthase F1 domain, revealing how ATP hydrolysis couples to mechanical rotation in 120-degree steps.

Structural transition of the ground-state structure to steady-state structures by sequential binding of ATP to V/A-ATPase

The paper identifies intermediate structures of V/A-ATPase during ATP activation using cryo-EM, showing how the ground-state structure transitions to steady-state through sequential ATP binding.

Autoinsertion (LAiR): rapid functional reconstitution of integral membrane proteins into lipid bilayers

This paper introduces LAiR, a method for rapidly reconstituting integral membrane proteins into lipid bilayers, showing improved activity, stability, and preservation of protein properties compared to standard techniques.

An actin-like filament from Clostridium botulinum exhibits a novel mechanism of filament dynamics

The paper identifies a ParM protein from Clostridium botulinum that forms a double-stranded filament with unique dynamics involving Pi-induced lateral shifts and distinct interactions with ParR.

Structural snapshots of V/A-ATPase reveal a new paradigm for rotary catalysis

The paper determines atomic models of V/A-ATPase catalytic intermediates, revealing a ratchet-like mechanism for unidirectional rotation instead of a power stroke model.

CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre

The paper shows CAMSAP2 nucleates microtubules from soluble β-tubulin without γ-tubulin, reducing the nucleation barrier and forming aster-like structures.

A novel capsid protein network allows the characteristic inner membrane structure of Marseilleviridae giant viruses

The paper investigates the structure of tokyovirus, a Marseilleviridae giant virus, revealing a novel capsid protein network that forms its characteristic inner membrane structure.

The mechanical inhibition of the isolated Vo from V-ATPase for proton conductance

The paper determines the cryo-EM structures of holo V/A-ATPase and isolated Vo to elucidate the auto-inhibited form of Vo and its regulation of proton conductance.