N. Sakai

Pigmentation level of human iPSC-derived retinal pigment epithelium cell does not indicate a specific gene expression profile

The paper analyzes the relationship between pigmentation level and gene expression in human iPSC-derived RPE cells, finding no specific gene expression profile correlates with color intensity.

Structural basis of Irgb6 inactivation by Toxoplasma gondii through the phosphorylation of switch I.

The paper reveals how Toxoplasma gondii inactivates Irgb6 through ROP18-mediated phosphorylation, preventing its function in host defense.

Structural model of microtubule dynamics inhibition by Kinesin-4 from the crystal structure of KLP-12 - tubulin complex

The paper reports the crystal structure of KLP-12-tubulin complex, revealing how KLP-12 inhibits microtubule dynamics by altering tubulin curvature.

Structural basis of membrane recognition of Toxoplasma gondii vacuole by Irgb6

This study determines the crystal structures of Irgb6 in GTP-bound and nucleotide-free forms, revealing a unique PVM binding interface that explains its membrane recognition mechanism.

POLArIS, a versatile probe for molecular orientation, revealed actin filaments associated with microtubule asters in early embryos

The paper introduces POLArIS, a probe for molecular orientation imaging, and demonstrates its use in visualizing actin filaments associated with microtubule asters in early embryos.

CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre

The paper shows CAMSAP2 nucleates microtubules from soluble β-tubulin without γ-tubulin, reducing the nucleation barrier and forming aster-like structures.

Inducing Human Retinal Pigment Epithelium-like Cells from Somatic Tissue

This study develops a simplified method to induce RPE-like cells from human fibroblasts and validates their properties using single-cell RNA sequencing.

Pharmacological inhibition of PRMT7 links arginine monomethylation to the cellular stress response

This study identifies a PRMT7 inhibitor and shows its effect on arginine methylation of stress proteins, linking PRMT7 to cellular stress response.

Morphological changes of plasma membrane and protein assembly during clathrin-mediated endocytosis

This study visualizes membrane morphology and protein dynamics during clathrin-mediated endocytosis in living cells, revealing actin's role in pit closure.