K. Namba

Use of phase plate cryo-EM reveals conformation diversity of therapeutic IgG with 50 kDa Fab fragment resolved below 6 angstrom

This study uses phase plate cryo-EM to reveal conformational diversity in therapeutic IgG, achieving sub-nanometer resolution of the Fab fragment.

Dimeric assembly of F1-like ATPase for the gliding motility of Mycoplasma

The paper presents a 3.2 Å resolution cryo-EM structure of a dimeric F1-like ATPase complex in Mycoplasma mobile, revealing its role in gliding motility through conserved rotary mechanisms.

Cryo-EM inspired NMR analysis reveals a pH-induced conformational switching mechanism for imparting dynamics to Betanodavirus protrusions

This study reveals a pH-induced conformational switching mechanism in Betanodavirus protrusions that governs infectivity through dynamic structural changes.

FliH and FliI help FlhA bring strict order to flagellar protein export in Salmonella

This study identifies the role of FlhA's GYXLI motif and the flagellar ATPase complex in regulating substrate specificity switching of the fT3SS during flagellar assembly in Salmonella.

Cryo-EM of cytoskeletal 'fibril' protein involved in Spiroplasma swimming

The paper determines the structure of a fibril protein in Spiroplasma using cryo-EM, revealing its helical organization and interactions with MreB filaments.

Development of a 1:1-binding biparatopic anti-TNFR2 antagonist by epitope selection

This study develops a biparatopic anti-TNFR2 antagonist that binds in a 1:1 ratio, preventing unwanted signal transduction and suppressing regulatory T cell proliferation.

The cryo-EM structure of the CENP-A nucleosome in complex with ggKNL2

The paper presents the cryo-EM structure of the chicken CENP-A nucleosome complexed with ggKNL2, revealing how ggKNL2 recognizes CENP-A and facilitates its deposition.

Conserved GYXLI motif of FlhA is involved in dynamic domain motions of FlhA required for flagellar protein export

This study identifies the conserved GYXLI motif in FlhA as critical for dynamic domain motions in FlhAC, enabling efficient flagellar protein export via the fT3SS.

Structure of cyanobacterial photosystem I complexed with Cytochrome c6 and Ferredoxin at 1.97 A resolution

The paper presents a high-resolution cryo-EM structure of cyanobacterial photosystem I complexed with Cytochrome c6 and Ferredoxin, revealing binding interactions and a proposed electron transfer mechanism.

Chained structure of dimeric F1-like ATPase in Mycoplasma mobile gliding machinery

This study determines the structure of a dimeric F1-like ATPase in Mycoplasma mobile gliding machinery, showing it resembles F1-ATPase and supports an evolutionary link.

Isolation and structure of the fibril protein, a major component of the internal ribbon for Spiroplasma swimming

This study isolates and characterizes the fibril protein, a key component of the helical ribbon in Spiroplasma, revealing its structural organization and role in cell helicity.

Native structure of flagellar MS ring is formed by 34 subunits with 23-fold and 11-fold subsymmetries

The paper determines the native structure of the bacterial flagellar MS ring, revealing it is 34-fold symmetric with 23-fold and 11-fold subsymmetries in its inner and middle regions.

The FlhA linker mediates flagellar protein export switching during flagellar assembly

The paper identifies FlhA's linker as a switch that regulates flagellar protein export specificity during assembly by interacting with FlhAC to control chaperone binding.

Cryo-EM structure of the MgtE Mg2+ channel pore domain in Mg2+-free conditions reveals cytoplasmic pore opening

The paper determines the cryo-EM structure of MgtE in Mg2+-free conditions, revealing cytoplasmic pore opening and kink motions in TM2 and TM5 helices.

Membrane voltage-dependent activation of the flagellar protein export engine

The paper investigates how membrane voltage influences flagellar protein export by showing that a voltage increase recovers export in the absence of ion gradients.

The FlgN chaperone activates the Na+-driven engine of the flagellar protein export apparatus

The paper identifies FlgN as a chaperone that activates the Na+-driven export mechanism in the absence of the ATPase complex in bacterial flagellar protein export.

Stator remodeling mechanism of Bacillus subtilis flagellar motor during biofilm development

This study shows that the MotPS stator complex in Bacillus subtilis is essential for efficient biofilm maturation, despite not affecting swimming motility.

The mechanical inhibition of the isolated Vo from V-ATPase for proton conductance

The paper determines the cryo-EM structures of holo V/A-ATPase and isolated Vo to elucidate the auto-inhibited form of Vo and its regulation of proton conductance.

Refined mechanism of Mycoplasma mobile gliding based on structure, ATPase activity, and sialic acid binding of machinery

This paper elucidates the gliding mechanism of Mycoplasma mobile by combining structural analysis, ATPase activity, and sialic acid binding studies.

Structure of the native supercoiled flagellar hook as a universal joint

This paper reports the native supercoiled structure of the bacterial flagellar hook at 3.1 Å resolution using cryoEM single particle analysis, revealing its dynamic universal joint mechanism.