High dimensional analyses of cells dissociated from cryopreserved synovial tissue
L. T. Donlin, D. A. Rao, K. Wei, K. Slowikowski, M. J. McGeachy, J. D. Turner, N. Meednu, F. Mizoguchi, M. Gutierrez-Arcelus, D. J. Lieb, J. Keegan, K. Muskat, J. Hillman, C. Rozo, E. Ricker, , M. B Brenner
This paper establishes a protocol for dissociating cryopreserved synovial tissue to obtain viable cells with preserved transcriptomes and surface markers, enabling high-dimensional analysis of rheumatoid arthritis pathology.
bioRxiv · Mar 19, 2018Immunology