M. Koutmos

Structural basis of S-adenosylmethionine-dependent Allosteric Transition from Active to Inactive States in Methylenetetrahydrofolate Reductase

The paper reports crystal structures of cMTHFR in active and inhibited states, revealing structural changes and AdoMet binding, and investigates phosphorylation sites in hMTHFR.

Recharacterization of RSL3 reveals that the selenoproteome is a druggable target in colorectal cancer

This study recharacterizes RSL3 as a broad selenoprotein inhibitor, showing its potential as a therapeutic target for colorectal cancer by inducing ferroptosis.

Structure of full-length cobalamin-dependent methionine synthase and cofactor loading captured in crystallo

This study determines the first high-resolution structure of full-length cobalamin-dependent methionine synthase and captures cobalamin loading, revealing insights into its dynamic domain rearrangements.

Mode of inhibition of RNase P by gambogic acid and juglone

This study identifies gambogic acid and juglone as inhibitors of PRORP1, with distinct mechanisms of action, and shows they also inhibit human mtRNase P.

Disease associated mutations in mitochondrial precursor tRNAs affect binding, m1R9 methylation and tRNA processing by mtRNase P

This study examines how disease-linked mutations in mitochondrial pre-tRNAs affect mtRNase P activity, revealing a link between tRNA processing defects and mitochondrial disease.

Kinetic mechanism of human mitochondrial RNase P

This study investigates the kinetic mechanism of human mitochondrial RNase P, showing that MRPP1/2 enhances catalytic activity and substrate affinity, and facilitates MRPP3 recognition and cleavage efficiency.

Interplay between substrate recognition, 5’ end tRNA processing and methylation activity of human mitochondrial RNase P

This study shows that mtRNase P subunits synergistically recognize, cleave, and methylate some mitochondrial pre-tRNAs, with MRPP1/2 methylation activity enhanced by MRPP3.