This paper designs universal Ebola virus vaccine candidates using hyperglycosylation to focus antibody responses on conserved epitopes, showing cross-neutralizing activity against multiple orthoebolavirus species.

bioRxiv · Oct 14, 2023 · PreprintImmunology

A monkeypox mRNA-lipid nanoparticle vaccine targeting virus binding, entry, and transmission drives protection against lethal orthopoxviral challenge

A. W. Freyn, C. Atyeo, P. L. Earl, J. L. Americo, G.-Y. Chuang, H. Natarajan, T. R. Frey, J. Gall, J. I. Moliva, R. Hunegnaw, G. Asthagiri Arunkumar, C. Ogega, A. Nasir, H. Bennett, J. Johnson, , B. Moss

This paper develops an mRNA-lipid nanoparticle vaccine targeting four conserved MPXV surface proteins, showing superior protection against VACV compared to MVA.

bioRxiv · Dec 17, 2022 · PreprintImmunology

A multispecific antibody prevents immune escape and confers pan-SARS-CoV-2 neutralization

J. Misasi, R. R. Wei, L. Wang, A. Pegu, C.-J. Wei, O. Oloniniyi, T. Zhou, J. I. Moliva, B. Zhao, M. Choe, E. S. Yang, Y. Zhang, M. Boruszczak, M. Chen, K. Leung, , N. J. Sullivan

This paper develops multispecific antibodies that neutralize all SARS-CoV-2 variants, including Omicron, by targeting multiple epitopes on the spike protein.

bioRxiv · Jul 29, 2022 · PreprintImmunology

Rapid and Durable Protection Against Marburg Virus with a Single-Shot ChAd3-MARV GP Vaccine

R. Hunegnaw, A. Honko, L. Wang, D. Carr, T. Murray, W. Shi, C. N. M. Dullan, K. E. Foulds, K. N. Agans, R. W. Cross, J. B. Geisbert, T. W. Geisbert, C. Cheng, A. Ploquin, D. A. Stanley, , N. J. Sullivan

A single-shot ChAd3-MARV vaccine provided rapid and durable protection against lethal Marburg virus infection in macaques, with antigen-specific antibodies correlating strongly with immunity.

bioRxiv · Dec 22, 2021 · PreprintImmunology

High-Throughput, Single-Copy Sequencing Reveals SARS-CoV-2 Spike Variants Coincident with Mounting Humoral Immunity during Acute COVID-19

S. H. Ko, E. Bayat Mokhtari, P. Mudvari, S. Stein, C. D. Stringham, D. Wagner, S. Ramelli, M. J. Ramirez-Benitez, J. R. Strich, R. T. Davey, T. Zhou, J. Misasi, P. D. Kwong, D. S. Chertow, N. J. Sullivan, E. A. Boritz

This study uses high-throughput single-copy sequencing to detect SARS-CoV-2 spike variants during acute COVID-19, revealing genetic adaptation coinciding with humoral immunity.

bioRxiv · Feb 21, 2021Microbiology

Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes

T. Zhou, I.-T. Teng, A. S. Olia, G. Cerutti, J. Gorman, A. Nazzari, W. Shi, Y. Tsybovsky, L. Wang, S. Wang, B. Zhang, Y. Zhang, P. S. Katsamba, Y. Petrova, B. B. Banach, , P. D. Kwong

The paper develops biotin-labeled SARS-CoV-2 spike molecular probes using structure-based design and purification methods, achieving high yields and enabling antibody characterization.

bioRxiv · Jun 22, 2020Immunology

A Platform Incorporating Trimeric Antigens into Self-Assembling Nanoparticles Reveals SARS-CoV-2-Spike Nanoparticles to Elicit Substantially Higher Neutralizing Responses than Spike Alone

B. Zhang, C. W. Chao, Y. Tsybovsky, O. M. Abiona, G. B. Hutchinson, J. I. Moliva, A. S. Olia, A. Pegu, E. Phung, G. Stewart-Jones, R. Verardi, L. Wang, S. Wang, A. Werner, E. S. Yang, , P. D. Kwong

This paper develops a nanoparticle platform for displaying trimeric antigens, showing that SARS-CoV-2 spike-LuS nanoparticles induce 25-fold higher neutralizing responses than spike alone.

bioRxiv · Jun 11, 2020Immunology