A streamlined and comprehensive protocol for the generation and multi-omic analysis of human monocyte-derived macrophages
Olivia Palmer, Laurent Perreard, Fred Kolling, Patricia Ann Pioli, Brittany A. Goods
This paper presents a streamlined protocol for generating and multi-omic analyzing human macrophages from frozen PBMCs, showing their phenotypic stability and compatibility with snRNA-seq.
BMC Biotechnology · Dec 29, 2025Peer-reviewedImmunology