Author

T. Skarina

University of Toronto

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Microbiology· 5 Synthetic Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Research Focus:host-pathogen & immune response· 3 Research Focus:chromatin & epigenetic regulation· 2 Assay:structural biology· 2 Organism:bacteria (other)· 1 Approach:biophysical modeling· 1 Research Focus:cell signaling & trafficking· 1 Organism:E. coli· 1

Frequent Co-Authors

A. Savchenko· 3 shared Alexei V. SavchenkoiD· 3 shared P. J. StogiosiD· 3 shared P. J. Stogios· 3 shared A. D'Souza· 1 shared A. W. Ensminger· 1 shared A. Wang· 1 shared A.-C. Gingras· 1 shared

Activity by Year

2021-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 17 mutual co-author links

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3 shared papersMicrobiology

A. Savchenko

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Author profile →

6 papers

Recent·Most cited

Structural analysis of 23S rRNA methylating enzyme Cfr reveals RNA binding determinants for methylation regiospecificity and antibiotic resistance

Michael Fruci, Annia Rodríguez Hernández, T. Skarina, Lou Ann Verellen, Kaitlyn Tsai, Johanna M. Virta, Danica Galonić Fujimori, Alexei V. Savchenko, P.J. Stogios

This study determines the crystal structure of Cfr, identifies key residues for methylation specificity, and links these to antibiotic resistance in pathogens.

ACS Infectious Diseases · Jan 30, 2026Peer-reviewedMicrobiology

Structural and functional analyses of SARS-CoV-2 Nsp3 and its specific interactions with the 5' UTR of the viral genome

Sofia Lemak, T. Skarina, Robert Flick, Deepak T. Patel, P. J. Stogios, Alexei V. Savchenko

This study analyzes the structural and functional interactions of SARS-CoV-2 Nsp3 with the 5' UTR of the viral genome, identifying key domains involved in binding and translocation.

Microbiology Spectrum · Jul 7, 2025Peer-reviewedMicrobiology

Functional and Structural Characterization of an IclR Family Transcription Factor for the Development of Dicarboxylic Acid Biosensors

Chester Pham, Mohamed A. Nasr, T. Skarina, Rosa Di Leo, David H. Kwan, Vincent J. J. Martin, P.J. Stogios, Radhakrishnan Mahadevan, Alexei V. Savchenko

This paper characterizes the PcaR transcription factor, solving its crystal structures and demonstrating its function as a dicarboxylic acid biosensor in yeast.

FEBS Journal · May 2, 2024Peer-reviewedSynthetic Biology

Functional diversification despite structural congruence in the HipBST toxin-antitoxin system of Legionella pneumophila

J. D. Lin, P. J. Stogios, K. T. Abe, A. Wang, J. MacPherson, T. Skarina, A.-C. Gingras, A. Savchenko, A. W. Ensminger

This study characterizes a conserved TA system in Legionella pneumophila, showing functional divergence from its E. coli counterpart despite structural similarity.

bioRxiv · Nov 26, 2022Microbiology

Molecular mechanism of plasmid-borne resistance to sulfonamides

M. Venkatesan, M. Fruci, L. A. Verellen, T. Skarina, N. Mesa, P. J. Stogios, A. Savchenko

This study identifies the molecular mechanism of intrinsic sulfonamide resistance in Sul enzymes through structural and functional analyses, revealing a modified active site that confers high resistance without fitness cost.

bioRxiv · Jun 30, 2022Microbiology

Structural and molecular rationale for the diversification of resistance mediated by the Antibiotic_NAT family

P. J. Stogios, E. Bordeleau, Z. Xu, T. Skarina, E. Evdokimova, S. Chou, D.-T. Luke, A. D'Souza, S. Patel, G. Dantas, G. Wright, A. Savchenko

The paper analyzes Antibiotic_NAT family enzymes to understand resistance diversification, finding environmental variants confer broad-spectrum resistance and likely ancestral relationships to clinical genes.

bioRxiv · Sep 10, 2021Microbiology