Author

P. Milon

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 3 Biochemistry· 2 Bioengineering· 1 Microbiology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 7 Organism:bacteria (other)· 4 Research Focus:protein folding & dynamics· 3 Assay:structural biology· 3 Research Focus:cell signaling & trafficking· 2 Assay:proteomics· 2 Approach:biophysical modeling· 1 Research Focus:host-pathogen & immune response· 1

Frequent Co-Authors

J. A. Nakamoto· 3 shared A. Fabbretti· 2 shared A. L. Konevega· 2 shared B. Hayes· 2 shared B. Tosun· 2 shared C. J. Kupitz· 2 shared C. Kulakman· 2 shared E. Ayan· 2 shared

Activity by Year

2019-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 24 mutual co-author links

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3 shared papersMicrobiology

J. A. Nakamoto

Mutual co-authors in cluster

Author profile →

7 papers

Recent·Most cited

4D Crystallography Captures Transient IF1-Ribosome Dynamics in Translation Initiation

I. Yapici, E. H. Dao, S. Yokoi, E. Destan, E. Ayan, A. Shafiei Kamel, F. B. Ertem, C. Kulakman, M. Yilmaz, B. Tosun, H. Ciftci, A. Kepceoglu, J. A. Johnson, O. Guven, A. Ergul, , H. DeMirci

This study uses 4D crystallography to capture transient dynamics of IF1 binding to the 30S ribosomal subunit, revealing allostery and interactions during translation initiation.

bioRxiv · Oct 27, 2023 · PreprintBiophysics

Ambient Temperature Bacterial Large Ribosomal Subunit Structure Enabled by Serial Femtosecond X-ray Crystallography

B. Tosun, Y. Rao, E. Destan, E. H. Dao, F. B. Ertem, M. Yilmaz, I. Yapici, M. Gul, E. Ayan, J. A. Johnson, A. Shafiei, C. Kulakman, B. Hayes, M. Liang, C. H. Yoon, , H. DeMirci

This study determines the structure of the 50S ribosomal subunit from Thermus thermophilus at ambient temperature using serial femtosecond X-ray crystallography, revealing new dynamics and antibiotic binding sites.

bioRxiv · Oct 24, 2023 · PreprintBiophysics

Orthoformimycin inhibits translation elongation by displacing the A-site tRNA and preventing peptide bond formation

A. Schedlbauer, T. Kaminishi, A. Fabbretti, P. Milon, X. Han, B. Ochoa-Lizarralde, R. Capuni, C. O. Gualerzi, S. R. Connell, P. Fucini

This study investigates how orthoformimycin inhibits translation elongation by displacing the A-site tRNA and blocking peptide bond formation using X-ray crystallography and kinetic analysis.

bioRxiv · Nov 29, 2021 · PreprintBiochemistry

(p)ppGpp inhibits 70S ribosome formation in Staphylococcus aureus by impeding GTPase-ribosome interactions

D. J. Bennison, J. A. Nakamoto, T. D. Craggs, P. Milon, J. B. Rafferty, R. M. Corrigan

This study shows (p)ppGpp inhibits 70S ribosome formation in Staphylococcus aureus by reducing RA-GTPase-ribosome interactions through inducing the OFF-state conformation.

bioRxiv · Jan 19, 2021 · PreprintMicrobiology

The complete, functional and dynamic cycle of the bacterial Initiation Factor 3

J. A. Nakamoto, R. Spurio, A. L. Konevega, A. Fabbretti, P. Milon

This study reveals the kinetic mechanisms and conformational changes of bacterial IF3 during translation initiation.

bioRxiv · Mar 16, 2019 · PreprintBiophysics

How the initiating ribosome copes with (p)ppGpp to translate mRNAs

D. S. Vinogradova, P. Kasatsky, E. Maksimova, V. Zegarra, A. Paleskava, A. L. Konevega, P. Milon

The paper investigates how the initiation factor IF2 senses the ppGpp to GTP ratio to regulate translation initiation in bacteria, showing that mRNA demand influences the competition between ppGpp and GTP for IF2 binding.

bioRxiv · Feb 10, 2019Biochemistry

DNA aptamers for the recognition of HMGB1 from Plasmodium falciparum

D. F. Joseph, J. A. Nakamoto, O. A. Garcia Ruiz, K. Penaranda, A. E. Sanchez-Castro, P. S. Castillo, P. Milon

This study identifies HMGB1 as a potential biomarker for malaria and develops DNA aptamers for its detection, showing high binding affinity and specificity.

bioRxiv · Jan 23, 2019Bioengineering