Author

R. A. Salido

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Microbiology· 3 Ecology· 2

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Assay:metagenomics· 4 Research Focus:benchmarking· 2 Assay:bulk RNA-seq· 1 Research Focus:cell type annotation· 1 Research Focus:ecology & biodiversity· 1 Research Focus:host-pathogen & immune response· 1 Organism:human· 1

Frequent Co-Authors

G. Humphrey· 4 shared R. Knight· 4 shared C. Marotz· 3 shared C. Martino· 3 shared J. P. Shaffer· 3 shared K. Sanders· 3 shared A. D. Swafford· 2 shared A. Gonzalez· 2 shared

Activity by Year

2020-2022 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 41 mutual co-author links

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4 shared papersMicrobiology

G. Humphrey

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Author profile →

5 papers

Recent·Most cited

A comparison of six DNA extraction protocols for 16S, ITS, and shotgun metagenomic sequencing of microbial communities

J. P. Shaffer, C. S. Carpenter, C. P. Martino, R. A. Salido, M. Bryant, K. Sanders, T. Schwartz, G. Humphrey, A. D. Swafford, R. Knight

The paper compares six DNA extraction protocols for 16S, ITS, and shotgun metagenomic sequencing, finding the MagMAX Microbiome kit performs best across multiple criteria.

bioRxiv · Mar 7, 2022Microbiology

Bacterial metatranscriptomes in wastewater can differentiate virally infected human populations

R. A. Salido, C. Martino, S. Karthikeyan, S. Huang, G. Rahman, A. Gonzalez, L. S. Zaramela, K. L. Beck, S. Bhute, K. Cantrell, A. P. Carrieri, S. Farmer, N. Haiminen, G. Humphrey, H.-C. Kim, , R. Knight

The paper shows that bacterial metatranscriptomes in wastewater can distinguish SARS-CoV-2 infected from uninfected human populations without directly detecting viral transcripts.

bioRxiv · Feb 23, 2022 · PreprintMicrobiology

Comparison of heat-inactivated and infectious SARS-CoV-2 across indoor surface materials shows comparable RT-qPCR viral signal intensity and persistence

R. A. Salido, V. J. Cantu, A. E. Clark, S. L. Leibel, A. Foroughishafiei, A. Saha, A. Hakim, A. Nouri, A. L. Lastrella, A. Castro-Martinez, A. Plascencia, B. Kapadia, B. Xia, C. Ruiz, C. Marotz, , R. Knight

This study compares RT-qPCR signal intensity and persistence of heat-inactivated and infectious SARS-CoV-2 on different surfaces, showing similar decay patterns and enabling simplified environmental monitoring protocols.

bioRxiv · Jul 16, 2021 · PreprintMicrobiology

Multi-omics profiling of Earth's biomes reveals patterns of diversity and co-occurrence in microbial and metabolite composition across environments

J. P. Shaffer, L.-F. Nothias, L. R. Thompson, S. G. Jon, R. A. Salido, S. P. Couvillion, A. D. Brejnrod, S. Huang, F. Lejzerowicz, N. Haiminen, H. L. Lutz, Q. Zhu, C. Martino, J. T. Morton, S. Karthikeyan, , St

The paper performs a multi-omics meta-analysis of microbial and metabolite data from diverse environments, revealing patterns of diversity and co-occurrence across biomes.

bioRxiv · Jun 4, 2021Ecology

A comparison of DNA/RNA extraction protocols for high-throughput sequencing of microbial communities

J. P. Shaffer, C. Marotz, P. Belda-Ferre, C. Martino, S. Wandro, M. Estaki, R. A. Salido, C. S. Carpenter, L. S. Zaramela, J. J. Minich, M. B. Bryant, K. Sanders, S. Fraraccio, G. Ackermann, G. Humphrey, , R. Knight

The paper compares DNA/RNA extraction protocols for microbial community sequencing, finding one protocol equivalent or better than a standard DNA-only method for RNA-based organisms.

bioRxiv · Nov 13, 2020Ecology