S. M. Markus

Cryo-EM Reveals the Mechanochemical Cycle of Reactive Full-length Human Dynein-1

The paper uses cryo-EM to study the mechanochemical cycle of full-length human dynein-1, revealing over 40 high-resolution structures and new mechanistic insights.

Conserved Roles for the Dynein Intermediate Chain and Ndel1 in Assembly and Activation of Dynein

This study identifies conserved roles of dynein intermediate chain and Ndel1 in dynein-dynactin-adapter complex assembly and activation through structural and functional analyses.

Microtubule binding-induced allostery promotes LIS1 dissociation from dynein prior to cargo transport

The paper investigates how microtubule binding affects LIS1 dissociation from dynein, showing that MT-bound dynein has low LIS1 affinity while MT-unbound dynein binds LIS1 strongly.

Zinc arrests axonal transport and displaces tau, doublecortin, and MAP2C from microtubules

The paper shows Zn2+ inhibits axonal transport by binding microtubules and displacing tau, DCX, and MAP2C, affecting motor protein movement.

The MAP She1 coordinates directional spindle positioning by spatially restricting dynein activity

The paper shows She1 restricts dynein activity in budding yeast to polarize spindle movement toward the daughter cell during mitosis.

Modeling disease-correlated TUBA1A mutation in budding yeast reveals a molecular basis for tubulin dysfunction

This study models a TUBA1A mutation in budding yeast to reveal how it disrupts microtubule function and dynein regulation, leading to spindle positioning defects.

Pac1/LIS1 promotes an uninhibited conformation of dynein that coordinates its localization and activity

The paper shows Pac1/LIS1 stabilizes an uninhibited conformation of dynein, enhancing its activity and coordination in cells.

Molecular basis for dyneinopathies reveals insight into dynein regulation and dysfunction

This study identifies molecular mechanisms of dyneinopathies using yeast to analyze disease-linked mutants and shows a correlation between dynein dysfunction severity and disease type.

EFFECTORS OF THE SPINDLE ASSEMBLY CHECKPOINT BUT NOT THE MITOTIC EXIT NETWORK ARE CONFINED WITHIN THE NUCLEUS OF SACCHAROMYCES CEREVISIAE

The paper shows that SAC effectors remain nuclear in yeast during mitosis, while MEN effectors move between nuclei in shared cytoplasm.