P. Chai

Cryo-EM Reveals the Mechanochemical Cycle of Reactive Full-length Human Dynein-1

The paper uses cryo-EM to study the mechanochemical cycle of full-length human dynein-1, revealing over 40 high-resolution structures and new mechanistic insights.

Cyclocarya paliurus polysaccharides improve high fat diet-fed mice lipid dysmetabolism through gut microbiota

This study shows Cyclocarya paliurus polysaccharides reduce obesity and lipid dysmetabolism in high fat diet-fed mice by modulating gut microbiota.

LRRC23 truncation impairs radial spoke 3 head assembly and sperm motility underlying male infertility

This study identifies LRRC23 as a critical component of the RS3 head in mammalian sperm, showing that its truncation leads to defective flagellar motility and male infertility.

RNA binding proteins and glycoRNAs form domains on the cell surface for cell penetrating peptide entry

Microtubule binding-induced allostery promotes LIS1 dissociation from dynein prior to cargo transport

The paper investigates how microtubule binding affects LIS1 dissociation from dynein, showing that MT-bound dynein has low LIS1 affinity while MT-unbound dynein binds LIS1 strongly.

Cryo-EM structure of an active central apparatus

The paper presents cryo-EM structures of Chlamydomonas reinhardtii central apparatus and its dynamic conformational behavior, revealing how it regulates ciliary beating.

Multi-curve fitting and tubulin-lattice signal removal for structure determination of large microtubule-based motors

The paper develops a method to determine high-resolution structures of large microtubule-based motors by removing tubulin-lattice signals from cryo-EM data without requiring microtubule reconstruction.

Structures of outer-arm dynein array on microtubule doublet reveal a motor coordination mechanism

The paper determines high-resolution structures of outer-arm dynein arrays bound to microtubule doublets, revealing a mechanism for motor coordination through ATP hydrolysis and tail-to-head interactions.

Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe

This study uses STED nanoscopy with a new dye to visualize mitochondrial cristae dynamics in live cells at high resolution.