C. L. Asbury

Architecture and flexibility of native kinetochores revealed by structural studies utilizing a thermophilic yeast

This study reveals the architecture and flexibility of native kinetochores using structural analyses of purified kinetochores from thermophilic yeast.

Kinetochores grip microtubules with directionally asymmetric strength

The paper shows that kinetochores grip microtubules six-fold stronger when pulled toward plus ends, suggesting directional asymmetry in attachment strength contributes to mitotic accuracy.

Mechanical coupling coordinates microtubule growth

The paper shows mechanical coupling coordinates microtubule growth in vitro using a dual laser trap assay, explaining coordination without free fit parameters.

Direct observation of coordinated assembly of individual native centromeric nucleosomes

The paper develops a microscopy assay to study centromeric nucleosome assembly, showing CENP-A can reach centromeres without HJURP but requires it for stable incorporation and AT-rich DNA for efficient deposition.

Working strokes produced by curling protofilaments at disassembling microtubule tips can be biochemically tuned and vary with species

The paper measures working strokes from curling protofilaments at disassembling microtubule tips, showing magnesium and species differences affect mechanical energy output.

Tension suppresses Aurora B kinase-triggered release of reconstituted kinetochore-microtubule attachments

The paper develops an assay to study Aurora B kinase's effect on kinetochore-microtubule attachments under tension, finding that tension suppresses Aurora B-triggered release.

Kinetochore-associated Mps1 regulates the strength of kinetochore-microtubule attachments via Ndc80 phosphorylation

This study shows Mps1 weakens kinetochore-microtubule attachments by phosphorylating Ndc80, contributing to error correction during mitosis.

The MAP She1 coordinates directional spindle positioning by spatially restricting dynein activity

The paper shows She1 restricts dynein activity in budding yeast to polarize spindle movement toward the daughter cell during mitosis.

The proteomic landscape of centromeric chromatin reveals an essential role for the Ctf19CCAN complex in meiotic kinetochore assembly

The paper uses proteomics to identify meiosis-specific kinetochore remodelling and reveals the essential role of the Ctf19cCCAN complex in meiotic kinetochore assembly.

XMAP215 and γ-tubulin additively promote microtubule nucleation in purified solutions

This study investigates whether XMAP215 and γ-tubulin synergize in microtubule nucleation and finds they act additively, not synergistically, in purified solutions.

The human Ska complex and Ndc80 complex interact to form a load-bearing assembly that strengthens kinetochore-microtubule attachments

This study shows the Ska complex directly strengthens kinetochore-microtubule attachments by forming a load-bearing connection with the Ndc80 complex and microtubule tips.

The unconventional kinetoplastid kinetochore protein KKT4 tracks with dynamic microtubule tips

This study identifies KKT4, a kinetoplastid kinetochore protein that binds and tracks with microtubule tips, enabling chromosome segregation despite lacking homology to known microtubule-binding domains.