Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair
Beeke Wienert, David N. Nguyen, Alexis Guenther, Sharon J. Feng, Melissa N. Locke, Stacia K. Wyman, Jiyung J. Shin, Katelynn R. Kazane, Georgia L. Gregory, Matthew Carter, Francis Wright, Bruce R. Conklin, Alexander Marson, Chris D. Richardson, Jacob E. Corn
The paper identifies CDC7 inhibition as a method to enhance HDR efficiency in CRISPR-Cas9 gene editing by inducing an early S-phase cell cycle arrest.
Nature Communications · Apr 30, 2020Peer-reviewedMolecular Biology