Stacia K. Wyman

Oligonucleotide synthesis errors are a source of untoward variation in HDR-mediated gene editing

This study identifies synthesis errors in ssODNs used for HDR-mediated gene editing and shows they propagate into the genome, potentially causing disease alleles.

Efficient in vivo mammalian neuron editing using peptide-mediated CRISPR enzyme delivery

This study develops Neuro-PERC, a peptide-mediated CRISPR delivery method, enabling efficient in vivo neuronal editing in mice and pigs, and extending survival in a Huntington's disease model.

Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) in primary human immune cells and hematopoietic stem cells

This paper introduces PERC, a method for efficient CRISPR editing in primary human immune cells and HSPCs using a peptide reagent, achieving over 90% editing efficiency without specialized equipment.

Identification of novel HPFH-like mutations by CRISPR base editing that elevates the expression of fetal hemoglobin

This study uses CRISPR base editing to identify novel mutations in the HBG promoter that increase fetal hemoglobin expression without harmful effects.

An integrated multi-omic analysis of iPSC-derived motor neurons from C9ORF72 ALS patients

The paper performs an integrated multi-omic analysis of iPSC-derived motor neurons from C9ORF72 ALS patients to identify dysregulated pathways and validate them in vivo and in postmortem data.

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair

The paper identifies CDC7 inhibition as a method to enhance HDR efficiency in CRISPR-Cas9 gene editing by inducing an early S-phase cell cycle arrest.

ATF4 mediates fetal globin upregulation in response to reduced β-globin

The paper identifies ATF4 as a key regulator of fetal globin re-expression in response to reduced β-globin, providing mechanistic insight into stress-induced globin compensation.

Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq

This paper introduces DISCOVER-Seq, a method to detect CRISPR off-targets in vivo with single-base resolution, applicable to multiple Cas nucleases.

High-level correction of the sickle mutation amplified in vivo during erythroid differentiation

Cas9-mediated correction of the sickle mutation in human hematopoietic stem cells achieves curative levels of editing.

Double Stranded DNA Damage and Genome Editing Trigger Ribosome Remodeling and Translational Shutdown

This study shows that DNA double-strand breaks cause ribosome remodeling and translational shutdown, independent of p53 and preceding transcriptional changes.

A most wanted list of conserved protein families with no known domains

The paper identifies and prioritizes conserved protein families with no known domains using metagenomics data and phylogenetic analysis.

Oligogenic inheritance of congenital heart disease involving a NKX2-5 modifier

The paper shows that a congenital heart defect can result from three inherited heterozygous missense variants in MYH7, MKL2, and NKX2-5.

Automated and accurate estimation of gene family abundance from shotgun metagenomes

This paper develops ShotMAP, a metagenome annotation pipeline, and evaluates its performance on marine and gut microbiome data, finding functional differences in Crohn's disease patients.