Beeke Wienert

Proximity determines donor candidacy during DNA double-stranded break homology directed repair

The paper develops RaPID-seq to study homology search during HDR and finds that DSB proximity determines template candidacy, with distant sequences not efficiently searched despite sequence identity.

Functional Screening in human HSPCs identifies optimized protein-based enhancers of Homology Directed Repair

The paper identifies optimized protein-based enhancers of HDR in human HSPCs through functional screening, improving HDR efficiency without increasing off-target effects.

Dual α-globin and truncated erythropoietin receptor knock-in restores hemoglobin production in α-thalassemia major-derived hematopoietic stem and progenitor cells

This paper describes a genome editing strategy that restores hemoglobin production in α-thalassemia major-derived hematopoietic stem and progenitor cells by integrating a functional α-globin gene and a truncated erythropoietin receptor.

Identification of novel HPFH-like mutations by CRISPR base editing that elevates the expression of fetal hemoglobin

This study uses CRISPR base editing to identify novel mutations in the HBG promoter that increase fetal hemoglobin expression without harmful effects.

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair

The paper identifies CDC7 inhibition as a method to enhance HDR efficiency in CRISPR-Cas9 gene editing by inducing an early S-phase cell cycle arrest.

Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq

This paper introduces DISCOVER-Seq, a method to detect CRISPR off-targets in vivo with single-base resolution, applicable to multiple Cas nucleases.