T. Hugel

Single-molecule FRET and tracking of transfected biomolecules: multi-dimensional protein dynamics in living cells

This paper introduces FRET-TTB, a method to track transfected biomolecules in living cells, showing it can measure conformational changes and localization with single-molecule resolution.

Cochaperones convey the energy of ATP hydrolysis for directional action of Hsp90

This study shows how three cochaperones couple ATP hydrolysis to directional conformational changes in Hsp90 during kinase maturation.

Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

This study evaluates the reliability and accuracy of single-molecule FRET for measuring protein structural dynamics and distances, finding high precision and accuracy with specific uncertainty limits.

Quantitative real-time in-cell imaging reveals heterogeneous clusters of proteins prior to condensation

The paper uses fluorescence microscopy to study protein clusters before condensation, finding dynamic clusters that are not freely diffusing and exist even in unstressed cells.

Inferring kinetic rate constants from single-molecule FRET trajectories - a blind benchmark of kinetic analysis tools

This study benchmarks eleven tools for inferring kinetic rate constants from smFRET trajectories using simulated and experimental data with varying challenges.

Autophosphorylation of the KaiC-like protein ArlH inhibits oligomerisation and interaction with ArlI, the motor ATPase of the archaellum

This study shows that autophosphorylation of ArlH inhibits its oligomerization and interaction with ArlI, essential for archaellum function and motility.

Hierarchical coupling between ATP hydrolysis and Hsp90's client binding site

The paper identifies a hierarchical mechanism by which ATP hydrolysis in Hsp90 influences its substrate binding site through structural asymmetry and allosteric dynamics.

Same Equilibrium. Different Kinetics. Protein Functional Consequences.

This study uses single-molecule FRET to show that Hsp90's ATPase function is regulated by different kinetic mechanisms, not just thermodynamics, and introduces the concept of functional stimulation through conformational confinement.

Proof-of-concept for the detection of early osteoarthritis pathology by endomicroscopy

This study demonstrates that early osteoarthritis pathology can be detected using existing clinical endomicroscopy technology by correlating cartilage stiffness and cellular spatial organization.

Effects of inhibitors on Hsp90’s conformational dynamics, cochaperone and client interactions

This study investigates how Hsp90 inhibitors affect its conformational dynamics and interactions with cochaperones and clients, finding minimal impact on global transitions and binding.