D. C. Lamb

Dissecting Mechanisms of Ligand Binding and Conformational Changes in the Glutamine-Binding Protein

This study investigates the ligand binding mechanism of GlnBP in E. coli, finding that conformational changes and ligand binding are correlated, and that the induced-fit mechanism is more consistent with the data than conformational selection.

Deep-Learning Assisted, Single-molecule Imaging analysis (Deep-LASI) of multi-color DNA Origami structures

Deep-LASI is a deep learning tool for rapid analysis of single-molecule imaging data, demonstrating accuracy and speed in FRET experiments and multi-color data.

mtHsp70 converts mitochondrial proteostasis distress into impaired protein import

This study identifies mtHsp70's role in regulating mitochondrial protein import efficiency during stress by showing its redistribution and reduced availability.

Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

This study evaluates the reliability and accuracy of single-molecule FRET for measuring protein structural dynamics and distances, finding high precision and accuracy with specific uncertainty limits.

Inferring kinetic rate constants from single-molecule FRET trajectories - a blind benchmark of kinetic analysis tools

This study benchmarks eleven tools for inferring kinetic rate constants from smFRET trajectories using simulated and experimental data with varying challenges.

Nanoscale organization of the endogenous ASC speck

This study uses super-resolution microscopy to analyze the nanoscale structure of endogenous ASC specks during NLRP3 inflammasome activation, revealing a dense core with extending filaments and changes in density during formation.

Orphan cytochrome P450 20A1 CRISPR/Cas 9 mutants and neurobehavioral phenotypes in zebrafish

This study creates zebrafish CYP20A1 mutants using CRISPR/Cas9 and finds behavioral differences suggesting a neurophysiological role for CYP20A1.

Structural basis of rotavirus RNA chaperone displacement and RNA annealing

This study identifies how the rotavirus NSP2 protein regulates RNA chaperone activity through structural changes that promote RNA annealing and release.

Tricarboxylic acid cycle and proton gradient in Pandoravirus massiliensis: Is it still a virus?

This study identifies functional enzymes of the tricarboxylic acid cycle in Pandoravirus massiliensis, suggesting it may possess energy production capabilities and challenging traditional virus definitions.

Substrate binding modulates the conformational kinetics of the secondary multidrug transporter LmrP

This study uses smFRET to show substrate binding modulates conformational kinetics of LmrP, revealing substrate-dependent structural changes in MFS transporters.

Dynamic interactions of type I cohesin modules fine-tune the structure of the cellulosome of Clostridium thermocellum

This study uses single-molecule techniques and simulations to show that cohesin-cohesin interactions and linker flexibility in scaffoldin proteins are critical for cellulosome structure and function.

Stability of local secondary structure determines selectivity of viral RNA chaperones

This study compares the ability of viral RNA chaperones NSP2 and σNS to promote inter-segment RNA-RNA interactions, finding that NSP2's high affinity for structured RNAs and efficient helix disruption enable successful inter-segment association, while σNS lacks this capacity.