C. Gebhardt

The Outer Kinetochore Proteins KNL-1 and Ndc80 complex are Required to Pattern the Central Nervous System

This study shows that KNL-1 and the NDC-80 complex are essential for proper axonal organization in C. elegans, revealing a non-canonical role for the KMN network in nervous system development.

Labelizer: systematic selection of protein residues for covalent fluorophore labeling

Labelizer systematically selects protein residues for covalent fluorophore labeling using a naive Bayes classifier and provides a tool and webserver for this purpose.

Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

This study evaluates the reliability and accuracy of single-molecule FRET for measuring protein structural dynamics and distances, finding high precision and accuracy with specific uncertainty limits.

Inferring kinetic rate constants from single-molecule FRET trajectories - a blind benchmark of kinetic analysis tools

This study benchmarks eleven tools for inferring kinetic rate constants from smFRET trajectories using simulated and experimental data with varying challenges.

Multi-parameter photon-by-photon hidden Markov modeling

This paper introduces multi-parameter H2MM to distinguish FRET dynamics from photophysical transitions in smFRET data, improving the analysis of biomolecular conformational changes.

Triggering closure of a sialic acid TRAP transporter substrate binding protein through binding of natural or artificial substrates

This study investigates the conformational changes in the sialic acid TRAP transporter SBP VcSiaP upon substrate binding, showing that substrate induces closure and provides structural insights for inhibitor development.

Cross-validation of distance measurements in proteins by PELDOR/DEER and single-molecule FRET

This study compares PELDOR/DEER and smFRET for measuring distances in proteins, identifying discrepancies caused by cryoprotectants and label-protein interactions.

Molecular and spectroscopic characterization of green and red cyanine fluorophores from the Alexa Fluor and AF series

The paper characterizes the molecular structures and spectroscopic properties of Alexa Fluor and AF series dyes using spectroscopy, mass spectrometry, and molecular dynamics simulations.

Intercalative DNA binding governs fluorescence enhancement of SYBR Gold

This study quantifies SYBR Gold binding to DNA using mechanical and spectroscopic methods, revealing intercalative binding and providing guidelines for quantitative DNA sensing.

Characterization of fluorescent proteins with intramolecular photostabilization

This study screens covalently-linked photostabilizers on fluorescent proteins, finding that azobenzene derivatives significantly enhance photostability compared to other stabilizers.