D. Grohmann

Structural basis of archaeal RNA polymerase transcription elongation and Spt4/5 recruitment

This study determines the structure of archaeal RNA polymerase and its interaction with Spt4/5, revealing conformational changes and a DNA-binding role for Spt5.

The transcriptional regulator EarA and intergenic terminator sequences modulate archaellation in Pyrococcus furiosus

This study identifies EarA as the master regulator of archaellum gene expression in Pyrococcus furiosus and shows intergenic terminators modulate archaellation.

Uncovering the temporal dynamics and regulatory networks of thermal stress response in a hyperthermophile using transcriptomics and proteomics

This study uses transcriptomics and proteomics to analyze the thermal stress response in Pyrococcus furiosus, revealing rapid transcriptome reprogramming and persistent protein upregulation during heat shock.

Transcriptional and translational dynamics underlying heat shock response in the thermophilic Crenarchaeon Sulfolobus acidocaldarius

This study investigates the heat shock response in Sulfolobus acidocaldarius using transcriptomic and proteomic analyses, revealing post-transcriptional and post-translational regulation mechanisms.

Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

This study evaluates the reliability and accuracy of single-molecule FRET for measuring protein structural dynamics and distances, finding high precision and accuracy with specific uncertainty limits.

Allosteric activation of CRISPR-Cas12a requires the concerted movement of the bridge helix and helix 1 of the RuvC II domain

This study investigates the role of helix 1 and the bridge helix in the allosteric activation of FnCas12a by showing their coordinated movement is essential for lid opening and DNA cleavage.

Probing the stability of the SpCas9-DNA complex after cleavage

This study investigates the stability of SpCas9-DNA complexes after cleavage using single-molecule magnetic tweezers, finding that post-cleavage states are more stable than pre-cleavage states.

Insights into rRNA processing and modification mapping in Archaea using Nanopore-based RNA sequencing

This study uses Nanopore sequencing to map rRNA processing and modifications in three archaeal species, revealing intermediates and dimethylation patterns.

Decoupling the bridge helix of Cas12a results in a reduced trimming activity and impaired conformational transitions

This study shows that modifying the bridge helix of Cas12a reduces its trimming activity and affects its conformational changes.

CopR, a global regulator of transcription to maintain copper homeostasis in Pyrococcus furiosus

The paper identifies CopR as a global regulator essential for copper homeostasis in Pyrococcus furiosus by analyzing transcriptome and genome-wide binding data.

Exploring prokaryotic transcription, operon structures, rRNA maturation and modifications using Nanopore-based native RNA sequencing

This study uses Nanopore-based native RNA sequencing to analyze prokaryotic transcriptomes, revealing operon structures, rRNA maturation, and modifications in E. coli, H. volcanii, and P. furiosus.

DNA origami-based single-molecule force spectroscopy unravels the molecular basis of RNA Polymerase III pre-initiation complex stability

The paper uses DNA origami force clamps to study how force affects initiation complex stability in RNA polymerase II and III systems, revealing Bdp1's critical role in RNAP III stability.