Direct In-Sample Sequencing of the 3' Transcriptome Expands the Capabilities of Optical Pooled Screens
Daniel J. Honigfort, Pedro Belda‐Ferre, David T. White, Kousik Sundararajan, Mariam Dawood, Jake A. LeVieux, Jacob Moreno, Xiaodong Qi, Kyle S. Metcalfe, Duuluu Naranbat, Andrew Altomare, Connor Thompson, Carlos Ruiz Perez, Bryan R. Lajoie, Hyuk-Jin Kwon, , Michael J. R. Previte
The paper introduces a platform that directly sequences 3'UTRs and guide RNAs in fixed cells alongside protein abundance and morphology, enabling CRISPR screening in lung cancer cells.
bioRxiv · Oct 11, 2025 · PreprintGenomics