Kyle S. Metcalfe

Direct In-Sample Sequencing of the 3' Transcriptome Expands the Capabilities of Optical Pooled Screens

The paper introduces a platform that directly sequences 3'UTRs and guide RNAs in fixed cells alongside protein abundance and morphology, enabling CRISPR screening in lung cancer cells.

A simplified hybrid capture approach retains high specificity and enables PCR-free workflow

This paper introduces a simplified hybrid capture method that eliminates PCR and bead-based steps while maintaining specificity and improving variant calling.

The use of iconPCR for 16S library preparation improves data quality and workflow

The paper introduces iconPCR, a novel PCR technology that improves data quality and workflow by ensuring uniform amplification across reactions, leading to more accurate microbial community analysis.

Utility Analyses of AVITI Sequencing Chemistry

This study compares the AVITI and Illumina sequencing chemistries for DNA and RNA short-read and long-read sequencing, finding AVITI has higher quality scores and lower error rates in short-read applications.