Kousik Sundararajan

Meiotic kinetochore reconstitution identifies chromosome segregation factors

This study reconstitutes vertebrate meiotic kinetochores to identify conserved factors essential for chromosome segregation in mammalian oocytes.

Direct In-Sample Sequencing of the 3' Transcriptome Expands the Capabilities of Optical Pooled Screens

The paper introduces a platform that directly sequences 3'UTRs and guide RNAs in fixed cells alongside protein abundance and morphology, enabling CRISPR screening in lung cancer cells.

DiMeLo-cito: a one-tube protocol for mapping protein-DNA interactions reveals CTCF bookmarking in mitosis

DiMeLo-cito improves DiMeLo-seq by eliminating wash steps, enabling mitotic protein-DNA interaction analysis in diploid human cells and revealing CTCF bookmarking.

Independence of centromeric and pericentromeric chromatin stability on CCAN components

This study shows that centromeric chromatin is mechanically resilient and distinct from pericentromeric chromatin, which deforms under force, with CCAN components not affecting pericentromere stretching.

Mapping protein-DNA interactions with DiMeLo-seq

The paper introduces DiMeLo-seq, a method for mapping protein-DNA interactions using long-read sequencing, and provides a protocol and analysis tool.

DiMeLo-seq: a long-read, single-molecule method for mappingprotein-DNA interactions genome-wide

DiMeLo-seq maps protein-DNA interactions genome-wide with single-molecule resolution by methylating DNA near binding sites and using long-read sequencing.

CENP-N promotes the compaction of centromeric chromatin

The paper shows CENP-N promotes centromere-specific nucleosome stacking and higher order chromatin structures in vitro and in cells.

Identification and characterization of centromeric sequences in Xenopus laevis

This study identifies and characterizes centromeric sequences in Xenopus laevis using CENP-A ChIP-seq and k-mer analysis, revealing diverse repeat sequences and mapping centromere positions on each chromosome.